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Merck

M8787

Magnesium chloride solution

PCR Reagent, 25 mM MgCI2 solution for PCR

Sinónimos:

PCR optimization

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Fórmula lineal:
MgCl2
Número CAS:
Peso molecular:
95.21
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
12352302
MDL number:
Form:
liquid
Servicio técnico
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Quality Level

form

liquid

packaging

vial of 1.5 mL

concentration

25 mM±1 mM

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture
diagnostic assay manufacturing

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

General description

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Application

Suitable for optimization of polymerase chain reactions.
Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression


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Clase de almacenamiento

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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