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Merck

MP0035

Kits LookOut® para detección de micoplasmas mediante PCR

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

Sinónimos:

PCR-based mycoplasma screening

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

Nombre del producto

Kits LookOut® para detección de micoplasmas mediante PCR, Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

quality

Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum

packaging

pkg of 1 kit

technique(s)

PCR: suitable

application(s)

detection
microbiology

compatibility

Optimized for use with JumpStart™ Taq DNA Polymerase, D9307.

storage temp.

2-8°C

Quality Level

100

Application

Vídeo del protocolo del kit de detección de micoplasmas por PCR
El kit de detección de micoplasmas por PCR LookOut® se ha utilizado para la detección selectiva de la infección por micoplasmas.

General description

El kit de detección de micoplasmas mediante PCR LookOut® utiliza la reacción en cadena de la polimerasa (PCR), que se ha establecido como el método de elección con la mayor sensibilidad en la detección de contaminación por Mycoplasma, Acholeplasma y Ureaplasma en cultivos celulares y otros productos biológicos procedentes de cultivos celulares. Se ha probado satisfactoriamente la capacidad del kit para amplificar el ADN genómico de Mycoplasma fermentans en concentraciones de 20 000 a 20 copias.
Los tubos de reacción incluidos con el kit vienen previamente recubiertos con los dNTP, los cebadores y el colorante de carga apropiados El tiempo de análisis total se reduce de manera notable en comparación con los protocolos generales que requieren la carga por separado de cada uno de los tubos de reacción.

Other Notes

El kit ha sido optimizado para su utilización con con el producto D9307. NO se recomienda ninguna ADN polimerasa con actividad 3’-5’ exonucleasa, que son la Tli, la Pfu y la Pwo. Las ADN polimerasas que pueden utilizarse son las Taq, Tfl y Tth.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Clase de almacenamiento

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
BCCN6452
BCCM7998
BCCM5625
BCCL4114
BCCL4119

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. What is the difference between Product No. MP0025 (VenorGem Mycoplasma Detection Kit) and Product No. MP0035 (Lookout Mycoplasma Detection Kit)?

    The assay kits have a similar range of species detected (see product insert for listing).  For Product No. MP0025, the components are supplied as individual reagents. For Product No. MP0035, the components are supplied in reaction tubes, already mixed and ready for addition of sample and Taq. The internal control for Product No. MP0035 is at 481 bp rather than 191 bp as found in Product No. MP0025.

  3. How many tests can be performed with Product MP0035, LookOut® Mycoplasma PCR Detection Kit?

    The kit contains 8 positive control reaction tubes and 24 test reaction tubes.  A positive control and negative control should be run with each assay.  Depending upon the number of samples run with each assay, the kit could test up to 24 samples.

  4. Which species of Mycoplasma will Product MP0035, LookOut® Mycoplasma PCR Detection Kit, recognize?

    Detection of all mycoplasma species tested so far and usually encountered as contaminants in cell cultures  This kit will detect A. laidlawii, M. agalactiae, M. arginini , M. arthritidis, M. bovis, M. cloacale, M. falconis, M. faucium, M. fermentans, M. hominis, M. hyorhinis, M. hyosynoviae, M. opalescens, M. orale, M. primatum, M. pulmonis, M. salivarium, M. spermatophilum, M. timone.  This kit is not suitable for detection of M. pneumoniae, U. urealyticum, or other clinically associated species.

  5. How much DNA is necessary for use with Product MP0035, LookOut® Mycoplasma PCR Detection Kit?

    Detection of Mycoplasma sp. requires less than 5 mycoplasma genomes per microliter of sample.  This is approximately 1-5 fg of DNA.

  6. When using Product MP0035, LookOut® Mycoplasma PCR Detection Kit, there is no band for the internal control?

    When a customer observes a band with the positive control and no band for the negative control, it is possible that the Taq could be the problem. We observe a lack of sensitivity with some polymerases from other manufacturers. The activity of the polymerases was enough to amplify the positive control but not the internal amplification control. The internal amplification control is the meter for the sensitivity of the PCR and the activity of the Taq used. The positive control contains more DNA and is good for result evaluation, only. We recommend that the customer use Jump Start Taq (Product No. D9307) as indicated in the protocol.

  7. Product MP0035, LookOut® Mycoplasma PCR Detection Kit does not contain TAQ.  What DNA polymerase should be used?

    The kit has been optimized for use with Product No. D9307. Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that may be used include Taq, Tfl, and Tth.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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