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Merck

O4264

Human Osteopontin

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Sinónimos:

Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)

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UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD03100698
Biological source:
human
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Mol wt:
60-65 kDa by SDS-PAGE (reducing)
Assay:
≥95% (SDS-PAGE)

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Nombre del producto

Osteopontin human, recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture

biological source

human

recombinant

expressed in NSO cells

sterility

sterile

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

60-65 kDa by SDS-PAGE (reducing)

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P10451

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Gene Information

human ... SPP1(6696)

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Analysis Note

Bioactivity Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is is 0.100-0.600 ug/mL.

Application

Anti-Osteopontin antibody produced in rabbit has been used for ELISA.

Biochem/physiol Actions

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

General description

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
MKCX0827
MKCW3524
MKCP5789
MKCM0838
MKCK9726

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Expanded comparative mapping between man
and rabbit and detection of a new conserved
segment between HSA22 and OCU4
C Chantry
Cytogenetic and genome research (2005)
Polycystic ovary syndrome is associated with increased osteopontin levels
European Journal of Endocrinology (2015)
Osteopontin Fragments with Intact Thrombin-Sensitive Site Circulate in Cervical Cancer Patients
Danny TM
PLoS ONE (2016)
Comparison of Plasma Osteopontin Levels between Patients with Borderline Ovarian Tumours and Serous Ovarian Carcinoma.
Zivny JH
Folia Biologica (Praha.) (2016)
Utility of Plasma Osteopontin Levels in Management of Community-Acquired Pneumonia
Jer-Hwa Chang
International Journal of Medical Sciences (2016)
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