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Merck

P1851

PIPES

BioPerformance Certified, suitable for cell culture

Sinónimos:

1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

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Fórmula empírica (notación de Hill):
C8H18N2O6S2
Número CAS:
Peso molecular:
302.37
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
227-057-6
Beilstein/REAXYS Number:
817713
MDL number:
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Quality Level

grade

BioPerformance Certified, Molecular Biology

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and Total Aerobic Microbial Count, tested

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless, soluble

application(s)

diagnostic assay manufacturing

foreign activity

DNAse, Exonuclease; NICKase, Endonuclease; RNAse; Protease, none detected

SMILES string

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

InChI key

IHPYMWDTONKSCO-UHFFFAOYSA-N

Application


  • Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics.: The use of PIPES buffer in this study was integral to maintaining the stability of immunoglobulin G during differential scanning fluorimetry. This method is crucial for evaluating the stability of antibody-based therapeutics (Moggridge et al., 2017).



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Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Leticia A Montoya et al.
Analytical chemistry, 86(12), 6032-6039 (2014-05-24)
Sulfhydryl-containing compounds, including thiols and hydrogen sulfide (H2S), play important but differential roles in biological structure and function. One major challenge in separating the biological roles of thiols and H2S is developing tools to effectively separate the reactivity of these
M Le Hir
Enzyme, 45(4), 194-199 (1991-01-01)
An impurity, probably an anion, present in some batches of the buffer substances 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), 2-morpholinoethane sulfonic acid (Mes) and piperazine-1,4-bis(2-ethane sulfonic acid (Pipes), activates the soluble 5'-nucleotidase from rat kidney. The affinity of the enzyme for 5'-IMP
Veronique Adoue et al.
Molecular systems biology, 10, 754-754 (2014-10-19)
Most complex disease-associated genetic variants are located in non-coding regions and are therefore thought to be regulatory in nature. Association mapping of differential allelic expression (AE) is a powerful method to identify SNPs with direct cis-regulatory impact (cis-rSNPs). We used