P3804
o-Fenilendiamina dihydrochloride
chromogenic, tablet
Sinónimos:
1,2-Phenylenediamine tablet, OPD, OPD Tablet
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Fórmula lineal:
C6H4(NH2)2·2HCl
Número CAS:
Peso molecular:
181.06
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
210-418-7
MDL number:
Beilstein/REAXYS Number:
3912045
Servicio técnico
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Permítanos ayudarleNombre del producto
o-Fenilendiamina dihydrochloride, tablet, 5 mg substrate per tablet
Quality Level
form
tablet
mp
258 °C (dec.) (lit.)
solubility
water: 1 tablet/10 mL, clear, colorless
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
General description
El diclorhidrato de o-fenilendiamina (OPD) es un sustrato cromógeno que puede utilizarse en procedimientos de enzimoinmunoanálisis de absorción (ELISA), ya que utiliza conjugados de peroxidasa de rábano picante. El sustrato rinde un producto final soluble que es de color naranja-marrón y puede leerse en el espectrofotómetro a 450 nm. La reacción del OPD puede detenerse con HCl 3N o H2SO4 3 M y leerse a 492 nm.
Application
o-Phenylenediamine dihydrochloride has been used:
- as a chromogenic substrate in indirect enzyme-linked immunosorbent assay (ELISA) to detect immunoglobin G (IgG) antibodies against certain recombinant antigens/proteins
- as a streptavidin-horseradish peroxidase (HRP) substrate sandwich ELISA to analyze human interleukin (IL)-17A, IL-23, and IP-10
- as a substrate in ELISA for determination of IgG-class antibodies to Mycobacterium bovis in serum
Packaging
16 mg total tablet weight
Preparation Note
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use.
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Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
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