P5538
Phosphoglucose Isomerase from Bacillus stearothermophilus
lyophilized powder, 300-1,000 units/mg protein
Sinónimos:
D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-603-1
MDL number:
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Permítanos ayudarleNombre del producto
Phosphoglucose Isomerase from Bacillus stearothermophilus, lyophilized powder, 300-1,000 units/mg protein
form
lyophilized powder
specific activity
300-1,000 units/mg protein
mol wt
189 kDa
composition
Protein, ≥60% biuret
storage temp.
−20°C
Quality Level
Application
Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. This product is from Bacillus stearothermophilus.
The enzyme from Sigma has been used in the determination of fructose 6-phosphate in a mutant strain of Rhizobium meliloti.
Biochem/physiol Actions
Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.
General description
The enzyme is part of the glycolytic pathway. Also, it is important in the industrial production of fructose 1,6-diphosphate (FDP) from glucose. The molecular mass is found to be approximately 189 kDa and it consists of four subunits, each with a molecular mass of approximately 50 kDa. Optimum pH is found to be between 9-10 and the isoelectric point is 4.2.
Other Notes
One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 9.0 at 30 °C.
Physical form
lyophilized powder containing Tris buffer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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A Arias et al.
Journal of bacteriology, 137(1), 409-414 (1979-01-01)
A mutant strain of complex phenotype was selected in Rhizobium meliloti after nitrosoguanidine mutagenesis. It failed to grow on mannitol, sorbitol, fructose, mannose, ribose, arabitol, or xylose, but grew on glucose, maltose, gluconate, L-arabinose, and many other carbohydrates. Assay showed
The Kinetics and Mechanism of a Reaction Catalyzed by Bacillus stearothermophilus Phosphoglucose Isomerase.
Widjaja A, et al.
Journal of Fermentation and Bioengineering, 86(3), 324-331 (1998)
Natsuko Miura et al.
Eukaryotic cell, 11(8), 1075-1082 (2012-07-04)
Glycolytic enzymes are cytosolic proteins, but they also play important extracellular roles in cell-cell communication and infection. We used Saccharomyces cerevisiae to analyze the secretory pathway of some of these enzymes, including enolase, phosphoglucose isomerase, triose phosphate isomerase, and fructose
Prashant Warang et al.
International journal of hematology, 96(2), 263-267 (2012-07-12)
Homozygous glucose phosphate isomerase (GPI) deficiency is one of the most important erythroenzymopathies causing hereditary non-spherocytic hemolytic anemia (HNSHA). We report an Indian patient with HNSHA showing 85 % reduction in GPI activity resulting from a homozygous missense replacement g.1459C
Sean D Schoville et al.
PloS one, 7(6), e40035-e40035 (2012-07-07)
The marine copepod Tigriopus californicus lives in intertidal rock pools along the Pacific coast, where it exhibits strong, temporally stable population genetic structure. Previous allozyme surveys have found high frequency private alleles among neighboring subpopulations, indicating that there is limited
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