P5542
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
lyophilized powder, ≥1,000 units/mg protein (Lowry)
Sinónimos:
1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-638-2
MDL number:
Servicio técnico
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lyophilized powder
Quality Level
specific activity
≥1,000 units/mg protein (Lowry)
foreign activity
Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein
storage temp.
−20°C
Gene Information
Bacillus cereus E33L ... BCZK3513(3026815)
General description
Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.
Application
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.
Biochem/physiol Actions
Used for the release of GPI anchored proteins from the membrane.
Physical form
Contains phosphate buffer salts, EDTA and stabilizer
Analysis Note
Acetylcholinesterase is measured according to Ellman, et al.
Other Notes
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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