P5994
Sustrato de fosfatasa
40 mg tablets
Sinónimos:
Fosfato de 4-nitrofenilo disodium salt hexahydrate, pNPP disodium salt hexahydrate
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
Acerca de este artículo
Fórmula lineal:
O2NC6H4OP(O)(ONa)2 · 6H2O
Número CAS:
Peso molecular:
371.14
NACRES:
NA.54
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleQuality Level
form
tablet
concentration
36-44 mg/tablet
impurities
p-nitrophenol, essentially free
solubility
H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)
storage temp.
−20°C
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
General description
Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.
Application
Sustrato cromógeno para usar en procedimientos ELISA de fosfatasa ácida y la alcalina. Rinde un producto final soluble que es de color amarillo y puede leerse espectrofotométricamente a 405 nm. La reacción de pNPP puede detenerse con NaOH 3 N y leerse a 405 nm.
Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.
Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).
Preparation Note
Contains 40 mg substrate per tablet
Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
Still not finding the right product?
Explore all of our products under Sustrato de fosfatasa
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Contenido relacionado
Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
Huang D, et al.
International journal of nanomedicine, 12(11), 7483-7483 (2017)
Tetsuya Harakuni et al.
Infection and immunity, 73(9), 5654-5665 (2005-08-23)
Noninvasive mucosal vaccines are attractive alternatives to parenteral vaccines. Although the conjugation of vaccine antigens with the B subunit of cholera toxin (CTB) is one of the most promising strategies for vaccine delivery to mucosal immune systems, the molecule cannot
Dissociation of bone resorption and bone formation in adult mice with a non-functional V-ATPase in osteoclasts leads to increased bone strength
Henriksen K, et al.
PLoS ONE, 6(11), e27482-e27482 (2011)