P7288
o-Fenilendiamina dihydrochloride
chromogenic, tablet
Sinónimos:
1,2-Phenylenediamine tablet, OPD, OPD Tablet
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Fórmula lineal:
C6H4(NH2)2·2HCl
Número CAS:
Peso molecular:
181.06
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
210-418-7
MDL number:
Beilstein/REAXYS Number:
3912045
Servicio técnico
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Permítanos ayudarleNombre del producto
o-Fenilendiamina dihydrochloride, tablet, 20 mg substrate per tablet
Quality Level
form
tablet
mp
258 °C (dec.) (lit.)
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
General description
El diclorhidrato de o-fenilendiamina (OPD) es un sustrato cromógeno que puede utilizarse en procedimientos de enzimoinmunoanálisis de absorción (ELISA), ya que utiliza conjugados de peroxidasa de rábano picante. El sustrato rinde un producto final soluble que es de color naranja-marrón y puede leerse en el espectrofotómetro a 450 nm. La reacción del OPD puede detenerse con HCl 3N o H2SO4 3 M y leerse a 492 nm.
Application
o-Phenylenediamine dihydrochloride has been used:
- as a substrate for the eosinophil peroxidase (EPO) assay in bronchoalveolar lavage fluid (BALF)
- in cell adhesion molecule-enzyme immunoassay (CAM-EIA) to analyze the amounts of E-selectin induced by test compounds (LPS or solutions and materials) on endothelial cells
- in ultra-sensitive enzyme-linked immunosorbent assay (ELISA) to measure prolactin and luteinizing hormone (LH)
Preparation Note
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
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Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Clase de almacenamiento
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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