P9172
Pyronin Y
for NA electrophoresis
Sinónimos:
Pyronin J, Pyronin Y, Pyronin G
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Fórmula empírica (notación de Hill):
C17H19ClN2O
Número CAS:
Peso molecular:
302.80
EC Number:
202-147-8
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3795789
Colour Index Number:
45005
Servicio técnico
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Molecular Biology
Quality Level
form
powder
composition
Dye content, ≥45%
technique(s)
nucleic acid detection: suitable
mp
250-260 °C (lit.)
solubility
water: 10 mg/mL, dark red
suitability
suitable for nucleic acid staining
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
InChI
1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
InChI key
INCIMLINXXICKS-UHFFFAOYSA-M
Application
Pyronin Y has been used:
- in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
- as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
- staining of CD34+ cells
Biochem/physiol Actions
Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
Preparation Note
This product is soluble in water 1 mg/ml, yielding a clear dark red solution.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Contenido relacionado
Gene expression differences between enriched normal and chronic myelogenous leukemia quiescent stem/progenitor cells and correlations with biological abnormalities
Affer M, et al.
Journal of Oncology, 2011 (2011)
Transcriptional profiling of mammary gland side population cells
Behbod F, et al.
Stem Cells, 24(4), 1065-1074 (2006)
Senescence and apoptosis block hematopoietic activation of quiescent hematopoietic stem cells with short telomeres
Wang J, et al.
Blood, 124(22), 3237-3240 (2014)