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Merck

R8758

Medio RPMI-1640

With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217820

Nombre del producto

Medio RPMI-1640, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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Application

El Medio RPMI-1640 se ha utilizado para el mantenimiento de líneas celulares y actúa como medio para diferentes células.

General description

El medio RPMI 1640 lo desarrolló el equipo de Moore en el Instituto Roswell Park Memorial en 1966. Se formuló una modificación del medio McCoy 5A para sostener las células linfoblastoides en cultivo en suspensión, pero desde entonces se ha demostrado que sirve de soporte para una amplia variedad de células que necesitan anclaje. Originalmente indicado para utilizar con un complemento sérico, se ha demostrado que el RPMI 1640 respalda diversas líneas celulares en ausencia de suero. También se ha utilizado ampliamente en protocolos de fusión y en el crecimiento de células híbridas. Este medio es adecuado para el cultivo de leucocitos humanos sanos y neoplásicos.

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

Clase de almacenamiento

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBM1129
RNBM1128
RNBM0239
RNBM0243
RNBM0103

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Does Product R8758, RPMI-1640 Medium, contain glucose?

    RPMI contains glucose at 2 g/L.  The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.

  3. Can I add the glutamine dipeptide to Product R8758, RPMI-1640 Medium?

    This formulation of RPMI already contains L-glutamine at 0.3 g/L.  It does not require further supplementation with L-glutamine or the L-glutamine dipeptide (Product No. G8541).

  4. Does Product R8758, RPMI-1640 Medium, contain HEPES?

    There is no HEPES in this formulation of RPMI.  We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.

  5. Can Product R8758, RPMI-1640 Medium, be frozen?

    We do not recommend freezing cell culture media. A precipitate may form upon thawing.  Also, this can cause a loss of amino acids, which may yield low cell growth.

  6. Does Product R8758, RPMI-1640 Medium, contain phenol red?

    This media contains phenol red.  The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Anders Abildgaard et al.
Psychoneuroendocrinology, 79, 40-48 (2017-03-05)
The gut microbiota has recently emerged as an important regulator of brain physiology and behaviour in animals, and ingestion of certain bacteria (probiotics) therefore appear to be a potential treatment for major depressive disorder (MDD). However, some conceptual and mechanistical
E L Davies et al.
Clinical and experimental immunology, 145(1), 183-189 (2006-06-24)
An increasing number of cell types, including peripheral blood mononuclear cells (PBMCs), have been demonstrated to release heat shock proteins (Hsps). In this paper we investigate further the hypothesis that Hsps are danger signals. PBMCs and Jurkat cells released Hsp70
Michelle J Nyhan et al.
BMC cancer, 16, 101-101 (2016-02-18)
Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
Samira Khiar et al.
Scientific reports, 7(1), 2561-2561 (2017-06-02)
The type I interferon response plays a pivotal role in host defense against infectious agents and tumors, and promising therapeutic approaches rely on small molecules designed to boost this system. To identify such compounds, we developed a high-throughput screening assay
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