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Merck

R9778

Anti-hnRNP-A1 antibody, Mouse monoclonal

~2 mg/mL, clone 4B10, purified from hybridoma cell culture

Sinónimos:

Anti-Heterogeneous Nuclear Ribonucleoprotein-A1

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UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD06798702

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Nombre del producto

Anti-hnRNP-A1 antibody, Mouse monoclonal, ~2 mg/mL, clone 4B10, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

4B10, monoclonal

form

buffered aqueous solution

mol wt

antigen 32-35 kDa

species reactivity

bovine, human, canine

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of HeLa cells

isotype

IgG2a

UniProt accession no.

P09651

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... HNRNPA1(3178)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-hnRNP-A1 antibody produced in mouse has also been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry.

Biochem/physiol Actions

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP-A1) is important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP-A1 from transportin. Higher expression is observed in proliferating and/or transformed cells than in differentiated tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-hnRNP-A1 (mouse IgG2a isotype) is derived from the 4B10 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from NZB mice immunized with purified human hnRNPA1. Heterogeneous nuclear ribonucleoprotein A1 (hnRNPs) consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa. hnRNP-A1 is ubiquitously expressed in cells and tissues.

Immunogen

purified human hnRNP-A1.

Physical form

Solution in 0.01 M phosohate buffered saline, pH 7.4, and 15 mM sodium azide.

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000223748
0000223748
0000207701
0000207701
055M4821V

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Youn-Jae Kim et al.
PloS one, 6(12), e28308-e28308 (2011-12-14)
Aberrant miR-21 expression is closely associated with cell proliferation, anti-apoptosis, migration, invasion, and metastasis in various cancers. However, the regulatory mechanism of miR-21 biogenesis is largely unknown. Here, we demonstrated that the tumor suppressor PTEN negatively regulates the expression of
hnRNP A1 nucleocytoplasmic shuttling activity is required for normal myelopoiesis and BCR/ABL leukemogenesis
Lervolino A, et al.
Molecular and Cellular Biology, 22(7), 2255-2266 (2002)
Functional diversity of the hnRNPs: past, present and perspectives
Han SP, et al.
The Biochemical Journal, 430(3), 379-392 (2010)
Blocking of an intronic splicing silencer completely rescues IKBKAP exon 20 splicing in familial dysautonomia patient cells
Bruun GH, et al.
Nucleic Acids Research, 46(15), 7938-7952 (2018)
Antisense Oligonucleotide Rescue of Deep-Intronic Variants Activating Pseudoexons in the 6-Pyruvoyl-Tetrahydropterin Synthase Gene.
Mart?-nez-Pizarro, et al.
Nucleic Acid Therapeutics, 32, 378-390 (2022)
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