RES3077T-A7
Tricine
Sinónimos:
Tricine, N-[Tris(hydroxymethyl)methyl]glycine
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Fórmula lineal:
(HOCH2)3CNHCH2CO2H
Número CAS:
Peso molecular:
179.17
EC Number:
227-193-6
UNSPSC Code:
12161700
NACRES:
NA.21
Beilstein/REAXYS Number:
1937804
MDL number:
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Permítanos ayudarleInChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
SMILES string
OCC(CO)(CO)NCC(O)=O
biological source
synthetic
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, microbial, and trace metals; tested
useful pH range
7.4-8.8
pKa (25 °C)
8.1
suitability
suitable for manufacturing use
foreign activity
Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
Quality Level
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Application
Buffer component for separation of low molecular weight peptides.
Tricine is a biological buffer often referred to as a “Good′s” buffer. The pKa of Tricine is 8.1 which makes Tricine is a good candidate for biological applications that require pH control above physiological. Tricine is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.
Tricine can be used in a wide array of biological applications including as a component in media formulations. Specific applications include buffers for electrophoresis, protein purification and diagnostic reagent production.
Tricine can be used in a wide array of biological applications including as a component in media formulations. Specific applications include buffers for electrophoresis, protein purification and diagnostic reagent production.
General description
Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.
M-Clarity Program
Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.
Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.
M-Clarity Program
Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.
Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.
Packaging
Product is available in the following package sizes:
RES3077T-A701X: 100 gm container
RES3077T-A702X: 1 kg container
RES3077T-A704X: 10 kg container
RES3077T-A705X: 25 kg container
RES3077T-A701X: 100 gm container
RES3077T-A702X: 1 kg container
RES3077T-A704X: 10 kg container
RES3077T-A705X: 25 kg container
Legal Information
SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany
Clase de almacenamiento
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in
Bin Deng et al.
PloS one, 9(7), e102362-e102362 (2014-07-19)
Maresins are a new family of anti-inflammatory and pro-resolving lipid mediators biosynthesized from docosahexaenoic acid (DHA) by macrophages. Here we identified a novel pro-resolving product, 13R,14S-dihydroxy-docosahexaenoic acid (13R,14S-diHDHA), produced by human macrophages. PCR mapping of 12-lipoxygenase (12-LOX) mRNA sequence in
Christian Nilsson et al.
Electrophoresis, 31(3), 459-464 (2010-02-02)
Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in
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