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Nombre del producto
Comprimidos de cóctel de inhibidores de proteasas SIGMAFAST™ sin EDTA, for use in purification of Histidine-tagged proteins
biological source
bovine lung
form
tablet
storage temp.
2-8°C
Quality Level
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Categorías relacionadas
Application
Biochem/physiol Actions
Features and Benefits
Pepstatina A: contienen una formulación patentada exclusiva, con una sal de pepstatina A para proporcionar una protección añadida que normalmente no se obtiene en un formato de comprimido.
Especificidad amplia: inhibe las proteasas de serina, cisteína y aspártico, y las metaloproteasas, proporcionando una amplia protección a las muestras proteicas.
Alto rendimiento: un comprimido produce 100 ml de la combinación, y se recomienda para la inhibición de las proteasas presentes en un máximo de 20 g de masa celular en 100 ml de extracto.
Estabilidad prolongada: los comprimidos son estables tal como se suministran durante un mínimo de 2 años a 2–8 °C, y la disolución de inhibidores de proteasa reconstituida (1´ o 10´) es estable por lo menos durante 2 semanas a 2–8 °C.
General description
Other Notes
Bestatina
E-64
Pepstatina A
Fosforamidon
Leupeptina
Aprotinina
Physical form
Preparation Note
Legal Information
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A
Clase de almacenamiento
8A - Combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the difference between Protease Inhibitors P8849 and S8830?
P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).
Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..
How should I dissolve Product No. S8830, SIGMAFAST™ Protease Inhibitor?
You can dissolve the product in 100 ml of water to make a 1X solution. You can make a 10X stock solution using 10 ml of water and then dilute 1:10 for use. Concentrations greater than 1X may appear slightly hazy. This will not affect the performance of the protease inhibitors.
Are there publications that cite use of Product S8830, SIGMAFAST™ Protease Inhibitor Cocktail Tablets?
Yes. Several examples of published papers that cite the use of Product S8830 include these:1. Smith, E.R., et al., Instability of fibroblast growth factor-23 (FGF-23): Implications for clinical studies. Clinica Chimica Acta, 412(11-12), 1008-1011 (2011). Use was with blood at 20 μL cocktail per 1 mL of blood.2. Reber, S., "Isolation of Centrosomes from Cultured Cells" (Chapter 8), from Microtubule Dynamics: Methods in Molecular Biology (Springer Protocols), vol. 777, pp. 107-116 (2011).
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