S8884

Safranin O

Name: Safranin O
Brand: SIGMA

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Sinónimos:

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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About This Item

Fórmula empírica (notación de Hill):

C₂₀H₁₉ClN₄

Número CAS:

477-73-6

Peso molecular:

350.84

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24899820

EC Number:

207-518-8

MDL number:

MFCD00011759

Colour Index Number:

50240

Beilstein/REAXYS Number:

3924099

Nombre del producto

Safranin O, certified by the BSC

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

agency

certified by the BSC

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

green to very dark green

solubility

H₂O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Categorías relacionadas

Clinical & Diagnostics

Dry Dyes & Certified Stains

Hematology Stains

Histology Stains

Tissue Diagnostics

Application

Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.

Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.

Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

General description

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

pictograms

GHS05

signalword

Danger

hcodes

H318

pcodes

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue

Sharma A, et al.

Bio-protocol, 9(4) (2016)

Paul A. Banaszkiewicz

Classic Papers in Orthopaedics (2014)

Perturbations in the HDL metabolic pathway predispose to the development of osteoarthritis in mice following long-term exposure to western-type diet

I.-E.Triantaphyllidou

Osteoarthritis and Cartilage / OARS, Osteoarthritis Research Society (2013)

Maria Dannessa Delost

Introduction to Diagnostic Microbiology for the Laboratory Sciences (2014)

Novel markers of osteogenic and adipogenic differentiation of human bone marrow stromal cells identified using a quantitative proteomics approach

CeciliaGraneli

Stem Cell Research (2014)

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