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Merck

T2391

Triosephosphate Isomerase from rabbit muscle

Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein

Sinónimos:

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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Número CAS:
9023-78-3
UNSPSC Code:
12352204
NACRES:
NA.54
Número CE:
5.3.1.1 (BRENDA, IUBMB)
MDL number:
MFCD00082080

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Nombre del producto

Triosephosphate Isomerase from rabbit muscle, Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein

biological source

rabbit muscle

type

Type III-S

form

ammonium sulfate suspension

specific activity

≥4,000 units/mg protein

mol wt

calculated mol wt 53.2 kDa

foreign activity

3-phosphoglyceric phosphokinase ≤0.01%
glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001%

storage temp.

2-8°C

Quality Level

200

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Analysis Note

Protein determined by biuret

Application

Triosephosphate isomerase has been used in a study to assess molecular characterizations of Cryptosporidium, Giardia, and Enterocytozoon. Triosephosphate isomerase has also been used in a study to investigate apoptosis of hepatocellular carcinoma cell lines.

Other Notes

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000294995
0000294995
0000294999
0000294999
0000294997

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Yi-Lin Sophia Chen et al.
Peptides, 36(2), 257-265 (2012-06-05)
Due to its malignancy, the development of effective therapeutic strategies for hepatocellular carcinoma (HCC) is of urgent needs. Natural antimicrobial peptides (AMPs), also known as host defense peptides (HDPs), not only act as direct antimicrobial agents, but also represent important
Beatty V Maikai et al.
Experimental parasitology, 131(4), 452-456 (2012-06-06)
The use of molecular diagnostic tools in epidemiological investigations of Cryptosporidium, Giardia, and Enterocytozoon has provided new insights into their diversity and transmission pathways. In this study, 157 stool specimens from 2-month to 70-year-old patients were collected, a polymerase chain
Susan K Boehlein et al.
The Plant journal : for cell and molecular biology, 99(1), 23-40 (2019-02-13)
Cereal yields decrease when grain fill proceeds under conditions of prolonged, moderately elevated temperatures. Endosperm-endogenous processes alter both rate and duration of dry weight gain, but underlying mechanisms remain unclear. Heat effects could be mediated by either abnormal, premature cessation
Rebecca Blomberg et al.
Nature, 503(7476), 418-421 (2013-10-18)
Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of
Tiia Kittilä et al.
Chembiochem : a European journal of chemical biology, 17(7), 576-584 (2016-01-12)
Nonribosomal peptide synthetases (NRPSs) produce many important and structurally complex natural products. Because of their architectures, reprogramming NRPSs has long been attempted to access new bioactive compounds. However, detailed characterization of NRPS catalysis and substrate selectivity by adenylation (A) domains
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