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Merck

V9264

Anti-Vinculin Antibody

mouse monoclonal, hVIN-1

Sinónimos:

Monoclonal Anti-Vinculin antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00162905

Nombre del producto

Anti-vinculina monoclonal antibody produced in mouse, clone hVIN-1, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

hVIN-1, monoclonal

form

buffered aqueous solution

mol wt

antigen 116 kDa

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.05-0.1 μg/mL using HS-68 total cell extract

isotype

IgG1

UniProt accession no.

P18206

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

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Categorías relacionadas

Biochem/physiol Actions

Marca de manera específica la vinculina en los contactos célula-célula y célula-sustrato. Reacciona fuertemente con la vinculina humana. Muestra reactividad cruzada con la metavinculina del músculo liso.
Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Immunogen

vinculina humana purificada del útero.

Application

Anti-Vinculin antibody, Mouse monoclonal has been used in
  • immunoblotting
  • immunofluorescence staining
  • immunocytochemistry
  • immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Clase de almacenamiento

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000487226
0000487227
0000487227
0000487226
0000444816

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Uncontrolled cell proliferation and cytoskeletal remodeling are responsible for tumor development and ultimately metastasis. A number of studies have implicated microRNAs in the regulation of cancer cell invasion and migration. Here, we show that miR-23b regulates focal adhesion, cell spreading
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