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Merck

Z4250

Zymosan A from Saccharomyces cerevisiae

for inducing inflamatory response

Sinónimos:

Yeast Cell Wall

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About This Item

Número CAS:
UNSPSC Code:
12352202
NACRES:
NA.61
MDL number:

Nombre del producto

Zymosan A from Saccharomyces cerevisiae, for inducing inflamatory response

biological source

Saccharomyces cerevisiae

form

powder with small lumps

technique(s)

flow cytometry: suitable
tissue culture: suitable

color

off-white to light brown

storage temp.

2-8°C

Quality Level

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Application

  • in flow cytometry
  • to study its effect on head kidney leukocytes from rainbow trout (Oncorhynchus mykiss)

Zymosan A from Saccharomyces cerevisiae has been used:
  • as a positive control to determine the activation of complement cascade
  • for respiratory burst (RB) assay
  • to assess its effects on performance, immunity, heat shock protein and apoptosis related gene expressions of dairy cows under heat stress (HS)
  • a stimulating agent in Krebs-Ringer phosphate (KRP) medium
  • to induce thermal hyperalgesia in mice

Biochem/physiol Actions

Zymosan is prepared from yeast cell wall and consists of protein-carbohydrate complexes. It is used to induce experimental sterile inflammation. In macrophages, zymosan-induced responses include the induction of proinflammatory cytokines, arachidonate mobilization, protein phosphorylation, and inositol phosphate formation. Zymosan A also raises cyclin D2 levels suggesting a role for the latter in macrophage activation besides proliferation. It potentiates acute liver damage after galactosamine injection suggesting that certain types of nonparenchymal cells other than Kupffer cells are involved in zymosan action. Zymosan A, a 13 beta glucan contributes to the development of organic dust-induced respiratory disease.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Certificados de análisis (COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the appearance of Product Z4250, Zymosan?

    The product is a powder which is described on the specification sheet.

  4. What is the particle size of Product Z4250, Zymosan?

    Sigma-Aldrich does not measure the particle size of zymosan. The average diameter of zymosan particles has been reported to be about 3 μm, as described on the product information sheet (under Documents, above), with the cited reference.

  5. How can I solubilize Zymosan, Product Z4250?

    Solubility information can be found in the product information sheet (under Documents, above) on our website.

  6. How can Product Z4250, Zymosan be prepared for use in a properdin assay?

    Information on preparation of zymosan for use in a properdin assay, which includes boiling zymosan suspensions, is described on the product information sheet (under Documents, above).

  7. Can Product Z4250, Zymosan suspension be autoclaved?

    We have no information as to whether zymosan suspensions can be autoclaved.  However, suspensions are stable to boiling conditions.

  8. What is the source of the Zymosan in Product Z4250?

    This zymosan is prepared from single cell yeast.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Impact of copper nanoparticles on porcine neutrophils: ultrasensitive characterization factor combining chemiluminescence information and USEtox assessment model
Pu Y, et al.
Materials Today Communications, 11, 68-75 (2017)
The G2A receptor controls polarization of macrophage by determining their localization within the inflamed tissue
Kern K, et al.
Frontiers in Immunology, 9, 2261-2261 (2018)
ACUTE INFLAMMATION AND RECOVERY IN RATS AFTER INTRATRACHEAL INSTILLATION OF A 1?3-
Young SH, et al.
Journal of Toxicology and Environmental Health. Part A, 64(4), 311-325 (2001)
PAMP induced expression of immune relevant genes in head kidney leukocytes of rainbow trout (Oncorhynchus mykiss)
Chettri JK, et al.
Developmental and Comparative Immunology, 35(4), 476-482 (2011)
Maria M M Kaisar et al.
PLoS biology, 16(4), e2005504-e2005504 (2018-04-19)
The molecular mechanisms through which dendritic cells (DCs) prime T helper 2 (Th2) responses, including those elicited by parasitic helminths, remain incompletely understood. Here, we report that soluble egg antigen (SEA) from Schistosoma mansoni, which is well known to drive

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Product Information Sheet

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