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1.37229

Inorganic Pyrophosphatase

EMPROVE® EXPERT

Synonym(s):

IPP, PPase

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About This Item

Specific activity:
≥100 units/mL
Assay:
≥95% (SDS-PAGE)
Biological source:
Escherichia coli
Recombinant:
expressed in E. coli
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biological source

Escherichia coli

Quality Segment

500

recombinant

expressed in E. coli

grade

GMP, Halal, Kosher

product line

EMPROVE® EXPERT

assay

≥95% (SDS-PAGE)

form

aqueous solution

specific activity

≥100 units/mL

mol wt

19.7 kDa

technique(s)

in vitro transcription (IVT): suitable

suitability

suitable for (In‑vitro transcription (IVT))

application(s)

pharma/biopharma processes
pharmaceutical

foreign activity

Low endotoxin
Low bioburden
DNase: none detected
RNase: none detected
Nickase: none detected

storage temp.

-10 to -25°C

General description

mRNA therapies represent a revolutionary approach to therapeutics such as vaccines, cancer treatments, and immunotherapies, offering novel ways to treat complex diseases. As a result, global interest in mRNA manufacturing has grown, while regulatory expectations have become increasingly exacting. In this environment, manufacturers require raw materials suitable for pharmaceutical use, ensuring the highest levels of quality and purity for successful mRNA production.

In response, we have developed the high-purity Enzyme Emprove® Expert portfolio, compliant with IPEC-PQG GMP standards, to ensure batch-to-batch consistency in your mRNA manufacturing processes. These critical raw materials are accompanied by comprehensive Emprove® dossiers, providing essential regulatory support to navigate the complexities of the biopharmaceutical industry while ensuring compliance and accelerating time to market.

As part of our SAFC® portfolio, these high-quality raw materials are designed to meet the specific needs of pharma and biopharma production.

Application

Inorganic pyrophosphatase (IPP) is an essential auxiliary enzyme used to enhance the efficiency of in vitro transcription (IVT) reactions for messenger RNA (mRNA) production. During IVT, inorganic pyrophosphate (PPi) accumulates as a reaction by-product and can inhibit RNA polymerase activity, ultimately reducing mRNA yield. IPP catalyzes the hydrolysis of PPi into inorganic phosphate, preventing product inhibition and supporting higher and more consistent transcription efficiency.

Features and Benefits

Our IVT enzyme Emprove® Expert IVT portfolio is designed to align with best-practice IVT methods for mRNA manufacturing and provides all purity requirements for reliable production of mRNA therapeutics and vaccines:
  • High purity (≥95%)
  • Low endotoxin and bioburden
  • DNase and RNase-free
Designed with therapeutic mRNA manufacturing in mind, our range of Emprove® Expert IVT enzymes provide all the quality attributes required for therapeutic mRNA raw materials, with validated quality control testing and manufacturing processes, certifications for AOF, Kosher, and Halal, and an extensive range of quality parameters provided in the COA.

Packaging

137229.0001 / 1 mL / plastic vial

137229.0010 / 10 mL / plastic bottle

137229.0050 / 50 mL / plastic bottle

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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