Quality Level
clone
M2, monoclonal
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
matrix
4% agarose bead; 45-165μm bead size
isotype
IgG1
capacity
≥0.6 mg/mL, gel binding capacity
shipped in
wet ice
storage temp.
−20°C
General description
EZview Red 抗-FLAG M2 亲和凝胶是含有抗FLAG M2抗体的树脂,共价交联在4%的琼脂糖珠上。该亲和凝胶用于将标记融合蛋白与样品结合,如细胞裂解物和组织,用于纯化标记蛋白,用于制备免疫沉淀分析。红色染料增强了可视性,以获得更有效的结果。琼脂糖珠结合于N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。
Application
Biochem/physiol Actions
适用于纯化N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。
Physical form
1:1 (v/v) 悬浮于含50%的甘油和15 ppm Kathon的PBS中
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
FLAG® 亲和凝胶,FLAG®标签,3xFLAG®标签,DYKDDDDK标签
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
采用M2单克隆抗体4%琼脂糖亲和凝胶进行的FLAG融合蛋白免疫沉淀(IP)实验方案
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
相关内容
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
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支持研究、治疗和疫苗生产的各种表达体系的蛋白质表达技术。
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