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Merck

F2426

EZview Red 抗-FLAG® M2 亲和凝胶

clone M2

别名:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352203
Clone:
M2, monoclonal
Technique(s):
affinity chromatography: suitable, immunoprecipitation (IP): suitable
Application:
IP, affinity chromatography
Citations:
204
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Quality Level

clone

M2, monoclonal

analyte chemical class(es)

proteins

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

matrix

4% agarose bead; 45-165μm bead size

isotype

IgG1

capacity

≥0.6 mg/mL, gel binding capacity

shipped in

wet ice

storage temp.

−20°C

General description

EZview Red 抗-FLAG M2 亲和凝胶是含有抗FLAG M2抗体的树脂,共价交联在4%的琼脂糖珠上。该亲和凝胶用于将标记融合蛋白与样品结合,如细胞裂解物和组织,用于纯化标记蛋白,用于制备免疫沉淀分析。红色染料增强了可视性,以获得更有效的结果。琼脂糖珠结合于N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。

Application

FLAG-和3xFLAG-标签融合蛋白的免疫沉淀(IP)。

洗脱-FLAG 多肽,甘氨酸,pH 3.5,3x FLAG 多肽

在我们的 FLAG® 应用门户了解更多产品详情。

Biochem/physiol Actions

适用于纯化N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。

Physical form

1:1 (v/v) 悬浮于含50%的甘油和15 ppm Kathon的PBS中

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

FLAG® 亲和凝胶,FLAG®标签,3xFLAG®标签,DYKDDDDK标签


存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

采用M2单克隆抗体4%琼脂糖亲和凝胶进行的FLAG融合蛋白免疫沉淀(IP)实验方案

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

相关内容

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

支持研究、治疗和疫苗生产的各种表达体系的蛋白质表达技术。

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