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L8384

亚油酸蛋白 来源于牛血清白蛋白

γ-irradiated, lyophilized powder, BioReagent, suitable for cell culture

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352202
Biological source:
bovine plasma
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Impurities:
≤50 EU/mg endotoxin
Concentration:
>0.5% (linolenic acid)
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biological source

bovine plasma

Quality Level

200

description

Contains 1.1-2.3 moles linoleic acid per mole BSA

sterility

γ-irradiated

product line

BioReagent

form

lyophilized powder

concentration

>0.5% (linolenic acid)

technique(s)

cell culture | mammalian: suitable

impurities

≤50 EU/mg endotoxin

solubility

water: 10 mg/mL, clear to hazy, colorless to light yellow

storage temp.

2-8°C

General description

Linoleic acid is an essential and highly consumed polyunsaturated fatty acid (PUFA). Albumin is a globular protein. Bovine serum albumin (BSA) is composed of a single polypeptide chain and cross-linked cysteine disulfide bridges. BSA is heart-shaped and is made up of three homologous domains I-III and each domain has two subdomains A and B.

Application

Linoleic Acid-Albumin from bovine serum albumin has been used: in cell culture and to study its effects on the human colonic adenocarcinoma cell line. It has also been used in the in vitro culture (IVC) of cow embryos/zygotes. The binding of linoleic acid to BSA improves its stability and solubility in culture media.

Biochem/physiol Actions

Linoleic acid plays a role in maintaining the transdermal water barrier in epidermis. Serum albumin constitutes about 52-62% of the total water-soluble fraction proteins in the blood plasma.

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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000456613
0000456613
SLCM4492
SLCD4647
SLBZ0308

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商品

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Frequently Asked Questions About Gamma-Irradiated FBS (Serum) and Other Products for Cell Culture

Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Takemasa Hidaka et al.
Theriogenology, 113, 176-182 (2018-03-20)
Variations in embryo production between individual oocyte donors represent a serious problem in cattle production, when implementing ovum pick-up (OPU) and in vitro maturation (IVM) of oocytes. However, the precise cause of this problem is unknown. Here, we aimed to investigate