Quality Level
sterility
sterile-filtered
form
liquid
storage condition
protect from light
technique(s)
cell culture | embryo: suitable
impurities
endotoxin, tested
components
HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
shipped in
ambient
storage temp.
2-8°C
Application
推荐用于室温下操作小鼠胚胎。M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的Krebs-Ringer碳酸氢盐溶液，与Whitten′s培养基非常相似。M16含有丙酮酸和乳酸作为能量来源，因为植入前胚胎不能有效利用葡萄糖。
M2培养基是对M16的进一步改良，用HEPES缓冲液替代一些碳酸氢盐。M2可用于在CO2培养箱外长时间收集和处理胚胎。
M2培养基是对M16的进一步改良，用HEPES缓冲液替代一些碳酸氢盐。M2可用于在CO2培养箱外长时间收集和处理胚胎。
Preparation Note
添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。
Analysis Note
溶液中的沉淀不影响本品性能。
该产品经过测试，能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80％。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
相关内容
Instructions
Hongzheng Sun et al.
Biology of reproduction, 100(3), 601-617 (2018-09-29)
Methionine adenosyltransferase II (MAT2A) is essential to the synthesis of S-adenosylmethionine, a major methyl donor, from L-methionine and ATP. Upon fertilization, zygotic genome activation (ZGA) marks the period that transforms the genome from transcriptional quiescence to robust transcriptional activity. During
Maryse Lessard et al.
Scientific reports, 9(1), 13829-13829 (2019-09-27)
The paternal environment is thought to influence sperm quality and future progeny may also be impacted. We hypothesized that prenatal exposure to environmentally-relevant contaminants impairs male reproduction, altering embryo gene expression over multiple generations. Folic acid (FA) can improve sperm
Takaya Totsuka et al.
Scientific reports, 10(1), 6556-6556 (2020-04-18)
To study the functional differences between maternal and paternal genomes in mammalian development, embryos with only one parental genome are often used. Androgenetic embryos are produced by the removal of maternal chromosomes before or after fertilization by techniques that require