General description
Sigma mirPremier microRNA分离试剂盒可以快速、有效地从不同的生物学来源样本，包括哺乳动物细胞培养物、动物组织、植物组织、微生物培养物中纯化和富集miRNA和其他小分子RNA，且无需进行任何有害的有机萃取。microRNA (miRNA) 属于小分子RNA，长度大约为21个核苷酸(nt)，通过各种方式调节着基因表达，包括翻译抑制、mRNA切割和脱腺苷化。此外，如果您关注信使RNA和其它大分子RNA，这种试剂盒还可用于分离总RNA。
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Application
mirPremier® microRNA Isolation Kit has been used to:
- prepare small RNA
- enrich small RNAfractions from the human astrocytes for small RNA sequencing and bioinformatics analysis
- isolate small RNA from Vigna mungo tissues
- extract miRNAs to detect the expression of miRNA-16
Features and Benefits
- Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples.
- Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications.
- Capable of extracting miRNA with high purity with no detectable large RNA.
- Hazardous organic extractions are not involved.
Legal Information
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
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仅试剂盒组分
产品编号
说明
- Binding Solution 2
- Lysis Solution
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
存储类别
10 - Combustible liquids
wgk
WGK 3
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Extraction of Small RNA and qPCR Validation of miRNAs in Vigna mungo
Paul S, et al.
Bio-protocol, 5(5), e1417-e1417 (2015)
Ying Zhang et al.
Genome biology, 18(1), 105-105 (2017-06-16)
Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying
Xiaoqiong Tang et al.
Cancer management and research, 12, 2119-2125 (2020-04-14)
LncRNA mortal obligate RNA transcript (MORT) is downregulated in different types of cancer, indicating its involvement in cancer biology. In this study, MORT and miRNA-16 were both downregulated in plasma of mantle cell lymphoma (MCL) patients than that in the