P0928
Resorufin pentyl ether
≥98% (TLC), Fluorimetric Cytochrome P450 substrate, powder
Synonym(s):
7-Pentyloxy-3-phenoxazone, 7-Pentyloxy-3H-phenoxazin-3-one, O7-Pentylresorufin, Pentoxyresorufin
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About This Item
Empirical Formula (Hill Notation):
C17H17NO3
CAS Number:
Molecular Weight:
283.32
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12161501
MDL number:
Beilstein/REAXYS Number:
8394939
Product Name
Resorufin pentyl ether,
assay
≥98% (TLC)
Quality Level
form
powder
solubility
acetonitrile: 0.95- 1.05 mg/mL, clear, orange
storage temp.
2-8°C
SMILES string
CCCCCOc1ccc2N=C3C=CC(=O)C=C3Oc2c1
InChI
1S/C17H17NO3/c1-2-3-4-9-20-13-6-8-15-17(11-13)21-16-10-12(19)5-7-14(16)18-15/h5-8,10-11H,2-4,9H2,1H3
InChI key
ZPSOKQFFOYYPKC-UHFFFAOYSA-N
General description
Resorufin pentyl ether is an analog of resazurin. Resorufin is a redox probe which is colorless and non-fluorescent in its reduced state. On oxidation, it fluoresces red. Resorufin is used widely as an indicator in microbiological media. It can be used to differentiate between actively metabolizing cells and inactive or dead cells.
Application
Resorufin pentyl ether has been used as a substrate to study the effects of Kaempferia parviflora extract on mouse hepatic cytochrome P450 enzymes.
Biochem/physiol Actions
Fluorimetric substrate for cytochrome P450 linked enzymes, CYP2B and CYP2B4.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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E S Tzanakakis et al.
Cell motility and the cytoskeleton, 48(3), 175-189 (2001-02-27)
Cultured rat hepatocytes self-assemble into three-dimensional structures or spheroids that exhibit ultrastructural characteristics of native hepatic tissue and enhanced liver-specific functions. The spheroid formation process involves cell translocation and changes in cell shape, indicative of the reorganization of the cytoskeletal
Advances in Microbial Ecology null
R B van Breemen et al.
Drug metabolism and disposition: the biological fate of chemicals, 26(2), 85-90 (1998-03-28)
An on-line mass spectrometric method has been developed to generate and identify drug metabolites formed by hepatic cytochromes P450. This method, pulsed ultrafiltration-mass spectrometry, may be used for rapid screening of drugs to determine their extent of metabolism by microsomal