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NE1039 Anti-IGFBP6 Mouse mAb (1H2-2D10)

NE1039
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the IGFBP6 protein in A431 cells.
Catalogue NumberNE1039
Brand Family Calbiochem®
SynonymsAnti-Insulin-Like Growth Factor Binding Protein 6
Application Data
Detection of human IGFBP6 by immunoblotting. Sample: Whole cell lysate from A431 cells. Priamry antibody: Anti-IGFBP6 Mouse mAb (1H2-2D10) (Cat. No. NE1039) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlA431 cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution
Biological Information
ImmunogenFull-length, recombinant human IGFBP6 (aa 1-241), expressed as a GST fusion protein
Clone1H2-2D10
HostMouse
IsotypeIgG2b
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NE1039 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Insulin-Like Growth Factor Binding Protein 6
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human IGFBP6 by immunoblotting. Sample: Whole cell lysate from A431 cells. Priamry antibody: Anti-IGFBP6 Mouse mAb (1H2-2D10) (Cat. No. NE1039) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the IGFBP6 protein.
BackgroundIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
HostMouse
ImmunogenFull-length, recombinant human IGFBP6 (aa 1-241), expressed as a GST fusion protein
Clone1H2-2D10
IsotypeIgG2b
Specieshuman
Positive controlA431 cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review