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ABC1392-100UG Anti-PDGF-C (GFD)

Anti-PDGF-C, Cat. No. ABC1392, is a rabbit polyclonal antibody that detects Platelet-derived growth factor C and has been tested for use in Western Blotting.

ABC1392-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABC1392-100UG
DescriptionAnti-PDGF-C (GFD)
Alternate Names
  • Platelet-derived growth factor C
  • Fallotein
  • Spinal cord-derived growth factor
  • SCDGF
  • VEGF-E
Background InformationPlatelet-derived growth factor C (UniProt: Q9NRA1; also known as PDGF-C, Fallotein, Spinal cord-derived growth factor, SCDGF, VEGF-E) is encoded by the PDGFC (also known as SCDGE, UNQ174/PRO200) gene (Gene ID: 56034) in human. PDGF-C is a disulfide-linked homodimeric growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. It also plays an important role in wound healing and angiogenesis. It also serves as a potent mitogen and chemoattractant for cells of mesenchymal origin. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form. Its CUB domain is localized to amino acids 46 to 163 and proteolytic removal of the CUB domain is essential for unmasking the receptor-binding epitopes of the core domain. PDGF-C is expressed in the fallopian tube, vascular smooth muscle cells in kidney, breast and colon and in visceral smooth muscle of the gastrointestinal tract. Highly expressed in retinal pigment epithelia. Its high expression is also reported in retinal pigment epithelia. In the kidney, it is constitutively expressed in parietal epithelial cells of Bowman's capsule, tubular epithelial cells, and in arterial endothelial cells. Higher levels of PDGF-C and have been reported in the malignant breast tumor microenvironment. In MCF-7 cells increased expression of PDGF-C is reported to enhance cell proliferation and tumor cell motility. Tissue plasminogen activator and matriptase are shown to process PDGF-C in MCF-7 cells to its active form. (Ref.: Hurst, NJ., et al. (2012). Biochem. J. 441(3); 909-918; Lei, H., and Kazlauskas, A (2008). Exp. Eye Res. 86(5); 711-712).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Applications
ApplicationAnti-PDGF-C, Cat. No. ABC1392, is a rabbit polyclonal antibody that detects Platelet-derived growth factor C and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Biological Information
ImmunogenA linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PDGFC
  • SCDGE
  • UNQ174/PRO200
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293T cells.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected PDGF-C in lysates from HEK293T cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC1392-100UG 04054839638527