608041
L-Lysine-13C6,15N2 hydrochloride
99 atom % 13C, 99 atom % 15N, 95% (CP)
Synonyme(s) :
SILAC Amino Acid, (S)-2,6-Diaminohexanoic acid-13C6,15N2 hydrochloride, 13C and 15N Labeled Lys, 13C and 15N Labeled lysine hydrochloride
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A propos de cet article
Formule linéaire :
H215N(13CH2)413CH(15NH2)13CO2H · HCl
Numéro CAS:
Poids moléculaire :
190.59
NACRES:
NA.12
PubChem Substance ID:
UNSPSC Code:
12352209
MDL number:
Isotopic purity:
99 atom % 13C, 99 atom % 15N
Assay:
95% (CP)
Mass shift:
M+8
Form:
solid
Quality Level
isotopic purity
99 atom % 13C, 99 atom % 15N
assay
95% (CP)
form
solid
optical activity
[α]25/D +20.7°, c = 2 in 5 M HCl
technique(s)
bio NMR: suitable, protein expression: suitable
mp
263-264 °C (dec.) (lit.)
mass shift
M+8
storage temp.
room temp
SMILES string
Cl.[15NH2][13CH2][13CH2][13CH2][13CH2][13C@H]([15NH2])[13C](O)=O
InChI
1S/C6H14N2O2.ClH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m0./s1/i1+1,2+1,3+1,4+1,5+1,6+1,7+1,8+1;
InChI key
BVHLGVCQOALMSV-GSMNGUNQSA-N
General description
Lysine is a basic ketogenic amino acid with a protonated alkyl amino group. Lysine degradation results in the formation of acetoacetate. Acetylation/deacetylation of lysine residues in histones is a mechanism to regulate chromatin organization in eukaryotes. Isotope labeled amino acids are required for the production of isotopically labeled proteins.
Application
- L-Lysine-13C6,15N2 hydrochloride has been used in pSILAC (pulsed stable isotope labeling by amino acids in cell culture) mass spectrometry to study protein profile in cells transfected with microRNAs.
- It has been used in SILAC isotopic labeling for the characterization of lysine racemase.
- It has been used in SILAC labeling for phosphoproteomic measurements in neuro-2a cells treated with and without GFKP-19 (2-pyrrolidone derivative).
Pulsed Stable Isotope labeling of amino acid has been used to study host-cell function, survival, the precise intracellular pathways in protein synthesis of HIV-1 infected human monocytederived macrophages.
Packaging
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach.
Ebner OA and Selbach M
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