939293
Protein delivery system
Human Chemerin, PODS®
Synonyme(s) :
PODS®
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NACRES:
NA.21
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Laissez-nous vous aidercolor
colorless
storage temp.
2-8°C
Quality Level
biological source
(Spodoptera frugiperda (Sf9) cell culture)
form
(monodisperse crystals)
packaging
1 ea of 25 μg
technique(s)
: suitable using 3D bioprinting | cell culture
impurities
<0.06 EU/mL Endotoxin
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General description
Protein delivery system, Human Chemerin, PODS, contains the polyhedrin protein co-crystalized with Human Chemerin. Chemerin, also known as tazarotene-induced gene 2 protein (TIG2), is a protein that is involved in immune regulation and has been implicated in various physiological and pathological processes. Chemerin is a chemoattractant adipokine that is expressed in white adipose, liver, skin, and lung tissues. Chemerin is a ligand for the G protein-coupled receptor chemokine-like receptor 1 (ChemR23), which is expressed on dendritic cells, macrophages, and adipocytes. Chemerin functions to recruit macrophages to sites of tissue damage and inflammation. Chemerin is also a regulator of glucose metabolism in the liver.
Application
PODS are composed of a bioactive cargo protein within a polyhedrin crystal lattice, with polyhedrin as the main protein. The 25 µg SKU size indicates the amount of bioactive cargo, typically 0.5% to 5% of the total protein. For instance, a 25 µg SKU at a 2% cargo loading contains 1.25 mg of total protein. PODS enable sustained release of cargo proteins when proteases degrade the polyhedrin scaffold. Growth factors are not bioavailable until released, and their concentration in media depends on the amount added and the release rate. In 10% serum, peak levels are usually reached within 24-48 hours, with about 20% of the cargo becoming soluble.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
PODS often require lower concentrations than conventional growth factors, as they maintain effective levels. Pre-incubating with serum or proteases enhances availability, while conventional factors can be added for immediate effects. Optimal amounts should be determined empirically.
Features and Benefits
- Protein delivery system, PODS Human Chemerin, comes with molecular weight of 21.7 kDa, length 188 aa, with endotoxin level of <0.06 EU/ml as measured by gel clot LAL assay. PODS co-crystals may be reconstituted at 200 million co-crystals/ml in sterile PBS. 20% glucose has a buoyant density closer to PODS co-crystals and can be useful for aliquoting.
- This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols. PODS co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).
- PODS provides durability, stability AND sustained release, addressing the limitations of conventional and enabling researchers to effortlessly control and manipulate cells in 2D and 3D culture as well as in-vivo.
Legal Information
PODS is a registered trademark of Cell Guidance Systems Ltd
Classe de stockage
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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