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Merck

05-386

Anti-Vinculin Antibody

UPSTATE®, mouse monoclonal, V284

Synonyme(s) :

Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL

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A propos de cet article

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
V284, monoclonal
Species reactivity:
human, rat, mouse, rabbit, chicken
Application:
immunohistochemistry
immunoprecipitation (IP)
western blot
Technique(s):
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Citations:
95
Uniprot accession no.:
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Nom du produit

Anti-Vinculin Antibody, clone V284, clone V284, Upstate®, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V284, monoclonal

species reactivity

human, rat, mouse, rabbit, chicken

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... VCL(7414)

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates of human A431 cells, lysates from chicken embryonic fibroblasts (CEF), or mouse 3T3/A31 fibroblasts

Application

Anti-Vinculin Antibody, clone V284 detects level of Vinculin & has been published & validated for use in IP, WB, IH.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Vinculin

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

117kDa

Immunogen

Human Vinculin

Physical form

Format: Purified
PBS, pH 7.4, containing 0.05% sodium azide and 1mg/ml BSA
Protein A chromatography

Preparation Note

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Paul G Leonard et al.
Nature chemical biology, 12(12), 1053-1058 (2016-10-25)
Despite being crucial for energy generation in most forms of life, few if any microbial antibiotics specifically inhibit glycolysis. To develop a specific inhibitor of the glycolytic enzyme enolase 2 (ENO2) for the treatment of cancers with deletion of ENO1
Unique morphology and focal adhesion development of valvular endothelial cells in static and fluid flow environments.
Butcher, JT; Penrod, AM; Garcia, AJ; Nerem, RM
Arteriosclerosis, Thrombosis, and Vascular Biology null
Zeinab Chitforoushzadeh et al.
Science signaling, 9(431), ra59-ra59 (2016-06-09)
Signal transduction networks coordinate transcriptional programs activated by diverse extracellular stimuli, such as growth factors and cytokines. Cells receive multiple stimuli simultaneously, and mapping how activation of the integrated signaling network affects gene expression is a challenge. We stimulated colon
Anuradha Illendula et al.
EBioMedicine, 8, 117-131 (2016-07-20)
Transcription factors have traditionally been viewed with skepticism as viable drug targets, but they offer the potential for completely novel mechanisms of action that could more effectively address the stem cell like properties, such as self-renewal and chemo-resistance, that lead
Temperature dependent specific heat capacity (Cp) of G-actin and talin or talin-vinculin bound to G-actin.
W H Goldmann et al.
Biochemical Society transactions, 20(3), 273S-273S (1992-08-01)

Numéro d'article de commerce international

RéférenceGTIN
05-38604053252268854

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