1.06177
TBE buffer
10 x pH 8.3 tris-borate-EDTA buffer
Synonyme(s) :
10X TBE running buffer, Tris-Borate EDTA buffer
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
41105319
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liquid
pH
8.2-8.4 (20 °C in H2O)
density
1.06 g/cm3 at 20 °C
storage temp.
15-25°C
General description
Tris, borate, and ethylenediaminetetraacetic (TBE) buffer is routinely used to conduct DNA and RNA agarose gel electrophoresis. It comprises a weak acid in neutral and anionic forms (e.g., COOH and COO- species) and a weak base, Tris, which is either in neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions buffer the pH, maintain a low conductivity medium, and transfer the electrical current. Ethylenediaminetetraacetic (EDTA) is used to chelate Mg2+ ions and inactivate the potential DNA nucleases. TBE is also used to perform a good resolution of DNA fragments that slightly enhances the separation of smaller fragments. It also protects nucleic acids from enzymatic degradation.
Analysis Note
Appearance (colour): almost colourless
Appearance (description): almost clear
pH-value: 8.2 - 8.4
Appearance (description): almost clear
pH-value: 8.2 - 8.4
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - Repr. 1B - Skin Sens. 1
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
hcodes
Classe de stockage
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
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Optical properties of buffers and cell culture media for optofluidic and sensing applications
Hoang VT, et al.
Applied Sciences, 9(6), 1145-1145 (2019)
Brian A Sanderson et al.
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric