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Merck

17-622

ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set

from rabbit, purified by using Protein A

Synonyme(s) :

Chip Antibody and primer set, H3K27me3 ChIP, H3K27me3, Histone H3 (tri methyl K27), Histone H3K27me3, Histone H3K27me3 ChIP

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.32
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, mouse
Application:
ChIP, WB
Citations:
196

Principaux documents

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biological source

rabbit

clone

polyclonal

purified by

using Protein A

species reactivity

human, mouse

manufacturer/tradename

ChIPAb+, Upstate®

technique(s)

ChIP: suitable, western blot: suitable

NCBI accession no.

NP_003484

UniProt accession no.

Q16695

shipped in

dry ice

Quality Level

100

Gene Information

human ... H3F3B(3021)

General description

17 kDa

Chaque jeu d'anticorps ChIPAb+ comprend des amorces témoins (testées sur chaque lot par PCR quantitative) vous permettant de valider biologiquement vos résultats d'immunoprécipitation dans un contexte locus-spécifique.
The ChIPAb+ Trimethyl-Histone H3 (Lys27) set includes the Anti-Trimethyl-Histone H3 (Lys27) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 139 bp region of human alpha-Satellite. The Trimethyl-Histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Trimethyl-Histone H3 (Lys27)-associated chromatin.
La méthylation des histones peut se produire sur deux résidus différents : l'arginine et la lysine. Selon le résidu méthylé, la méthylation de l'histone peut être associée à l'activation ou à la répression transcriptionnelle. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human Histone H3.
Épitope : Trimethyl Lys27

Application

&&

μ



μ
Domaine de recherche
Épigénétique et fonction nucléaire
Sous-domaine de recherche
Histones

Biochem/physiol Actions

Réactivité croisée à prévoir avec un large spectre d'espèces.
trimethyl-Histone H3 (Lys27)

Packaging

μ

Physical form

Anti-trimethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). μμ °

μμ °

μμ °

GAT GGT TCA ACA CTC TTA CA

Preparation Note

À -20 °C pendant 1 an à compter de la date d'expédition.

Analysis Note


μ®


μμ À conserver à -20 °C.

Amorce sens :

GAT GGT TCA ACA CTC TTA CA

Other Notes





Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Classe de stockage

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Genome-wide bovine H3K27me3 modifications and the regulatory effects on genes expressions in peripheral blood lymphocytes.
He, Y; Yu, Y; Zhang, Y; Song, J; Mitra, A; Zhang, Y; Wang, Y; Sun, D; Zhang, S
Testing null
Transgene- and locus-dependent imprinting reveals allele-specific chromosome conformations.
Lonfat, N; Montavon, T; Jebb, D; Tschopp, P; Nguyen Huynh, TH; Zakany, J; Duboule, D
Proceedings of the National Academy of Sciences of the USA null
Modular utilization of distal cis-regulatory elements controls Ifng gene expression in T cells activated by distinct stimuli.
Balasubramani, A; Shibata, Y; Crawford, GE; Baldwin, AS; Hatton, RD; Weaver, CT
Immunity null
Epstein-Barr virus nuclear antigens 3C and 3A maintain lymphoblastoid cell growth by repressing p16INK4A and p14ARF expression.
Maruo, S; Zhao, B; Johannsen, E; Kieff, E; Zou, J; Takada, K
Proceedings of the National Academy of Sciences of the USA null
Claudio D'Addario et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(3), 1069-1075 (2010-11-26)
Ethanol alters neural activity through interaction with multiple neurotransmitters and neuromodulators. The endogenous opioid system seems to play a key role, since the opioid receptor antagonist naltrexone (ReVia®) attenuates craving for alcohol. We recently reported that ethanol and acetaldehyde, the
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Numéro d'article de commerce international

RéférenceGTIN
17-62204053252328732

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