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Merck

178467

Anti-Apolipoprotein B Goat pAb

liquid, Calbiochem®

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A propos de cet article

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human
Application:
Citations:
11
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biological source

goat

antibody form

diluted serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting
Immunoelectrophoresis
Immunoprecipitation (Becker titer, 21 mg/mL))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

General description

Anti-Apolipoprotein B, goat polyclonal, recognizes human apolipoprotein B. It is validated for Western blotting, immunoelectrophoresis, and immunoprecipitation.
Goat polyclonal antibody supplied as diluted serum that has been defibrinated, delipidized, and adsorbed by solid phase chromatography. Recognizes apolipoprotein B.
Recognizes apolipoprotein B.

  • Antibody Target Gene Symbol: APOB
  • Target Synonym: Aa1064, Ac1-060, AI315052, ApoB-100, APOB-29, ApoB-48, APOLIPOPROTEIN B, APOLIPOPROTEIN B100, FLDB, LDLCQ4, MGC176318
  • Entrez Gene Name: apolipoprotein B (including Ag(x) antigen)
  • Hu Entrez ID: 338 (Related Antibodies: 178446, 178444)
  • Mu Entrez ID: 238055
  • Rat Entrez ID: 54225

Immunogen

Human
purified human, apolipoprotein B

Application

Immunoblotting (see application references)

Immunoelectrophoresis (see comments)

Immunoprecipitation (Becker titer, ≥21 mg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

Serum diluted in 500 mM NaCl, 50 mM Tris-HCl, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Monospecific as determined by immunoelectrophoresis (IEP) against 2X pooled human serum and neat pooled human plasma. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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David B Melville et al.
The Journal of biological chemistry, 295(25), 8401-8412 (2020-05-03)
Vesicles that are coated by coat protein complex II (COPII) are the primary mediators of vesicular traffic from the endoplasmic reticulum to the Golgi apparatus. Secretion-associated Ras-related GTPase 1 (SAR1) is a small GTPase that is part of COPII and
Tanja Kosenko et al.
The Journal of biological chemistry, 288(12), 8279-8288 (2013-02-13)
Proprotein convertase subtilisin/kexin type-9 (PCSK9) is a secreted protein that binds to the epidermal growth factor-like-A domain of the low density lipoprotein receptor (LDLR) and mediates LDLR degradation in liver. Gain-of-function mutations in PCSK9 are associated with autosomal dominant hypercholesterolemia
Yuehui Wang et al.
PloS one, 4(12), e8442-e8442 (2009-12-31)
A small fraction of dietary protein survives enzymatic degradation and is absorbed in potentially antigenic form. This can trigger inflammatory responses in patients with celiac disease or food allergies, but typically induces systemic immunological tolerance (oral tolerance). At present it
David Melville et al.
Molecular biology of the cell, 30(3), 387-399 (2018-11-30)
COPII-coated vesicles are the primary mediators of ER-to-Golgi trafficking. Sar1, one of the five core COPII components, is a highly conserved small GTPase, which, upon GTP binding, recruits the other COPII proteins to the ER membrane. It has been hypothesized
Enrico D Perini et al.
Traffic (Copenhagen, Denmark), 15(12), 1366-1389 (2014-10-01)
Early endosomes are organized in a network of vesicles shaped by cycles of fusion, fission, and conversion to late endosomes. In yeast, endosome fusion and conversion are regulated, among others, by CORVET, a hexameric protein complex. In the mammalian endocytic

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