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Merck

203391

(−)-Blebbistatin

≥98% (HPLC), solid, ATPase inhibitor, Calbiochem®

Synonyme(s) :

(-)-Blebbistatin

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A propos de cet article

Formule empirique (notation de Hill) :
C18H16N2O2
Numéro CAS:
856925-71-8
Poids moléculaire :
292.33
UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
MFCD08460907

Principaux documents

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Nom du produit

(-)-Blebbistatin, The active enantiomer of (±)-Blebbistatin that accounts for the inhibitory activity towards ATPase and myosin II-dependent cellular processes.

SMILES string

N2(CC[C@@]3(C2=Nc4c(cc(cc4)C)C3=O)O)c1ccccc1

InChI

1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3/t18-/m1/s1

InChI key

LZAXPYOBKSJSEX-GOSISDBHSA-N

assay

≥98% (HPLC)

form

solid

potency

2 μM IC50

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

methanol: 1.5 mg/mL
100% DMSO: 100 mg/mL
90% DMSO: 75 mg/mL

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Catégories apparentées

Biochem/physiol Actions

Cell permeable: yes
Product does not compete with ATP.
Reversible: yes

Disclaimer

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Other Notes

Eddinger, T.J. et al. 2007. J. Pharmacol. Exp. Ther.320, 865.
Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month in 90% DMSO or methanol (-20°C). Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

General description

The active enantiomer of (±)-Blebbistatin (Cat. No. 203390) that accounts for the inhibitory activity towards ATPase (IC50 = ~2 µM) and myosin II-dependent cellular processes. Also reported to potently inhibit the actomyosin ATPase activities of expressed smooth muscle myosin IIA (SMA) and smooth muscle myosin IIB (SMB) smooth muscle myosin II heavy-chain isoforms (IC50 ~3 µM).

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

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Dennis J Yuan et al.
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Journal of cell science, 135(14) (2022-06-16)
The metastatic progression of cancer remains a major issue in patient treatment. However, the molecular and cellular mechanisms underlying this process remain unclear. Here, we use primary explants and organoids from patients harboring mucinous colorectal carcinoma (MUC CRC), a poor-prognosis
Adam Germain et al.
Oncology, 102(6), 515-524 (2023-11-27)
Cancers in general, and specifically lung cancer, continue to have low patient survival rates when the patient is at an advanced stage when diagnosed. It appears that the local environment, especially fibroblasts and their signaling molecules, tends to induce metastasis
Cell clusters adopt a collective amoeboid mode of migration in confined nonadhesive environments.
Pag??s, et al.
Science Advances, 8, eabp8416-eabp8416 (2022)
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