219343
Cathepsin S, Human, Recombinant, E. coli
Cathepsin S, Human, Recombinant, E. coli, is prepared without a tag or fusion protein. A major lysosomal cysteine proteinase with high specific activity.
Synonyme(s) :
Cathepsin S Protein
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human
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥30,000 mU/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
technique(s)
cell based assay: suitable
UniProt accession no.
shipped in
wet ice
storage temp.
−70°C
Quality Level
Gene Information
human ... CTSS(1520)
General description
Note: 1 mU = 1 milliunit.
Research area: Cell Signaling
Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase that is preferentially expressed in macrophages and microglia. Shown to be involved in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenting cells by mediating invariant (Ii) chain degradation. Inhibition of cathepsin S results in impaired antigen-presentation.
Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase that is preferentially expressed in macrophages and microglia. Shown to be involved in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenting cells by mediating invariant (Ii) chain degradation. Inhibition of cathepsin S results in impaired antigen-presentation.
Application
Cathepsin S, Human, Recombinant, E. coli has been used in cathepsin digestion reactions.
Biochem/physiol Actions
Cathepsin S is a cysteine protease that plays a role in degradation of class II complexes in human B lymphocytes. It possesses endoproteolytic activity and aids in processing and presenting antigens to immune cells, thereby influencing the immune response. Cathepsin S is involved in facilitating the degradation of damaged or unwanted proteins within the endo-lysosomal pathway. Dysregulation of Cathepsin S activity leads to the development of various diseases such as arthritis, cancer, and cardiovascular diseases.
Physical form
In 35 mM MES, 35 mM sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.4.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Liuzzo, J.P., et al. 1999. Mol. Med.5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest.101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest.102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol.244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys.299, 334.
Kirschke, H., et al. 1989. Biochem. J.264, 467.
Riese, R.J., et al. 1998. J. Clin. Invest.101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest.102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol.244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys.299, 334.
Kirschke, H., et al. 1989. Biochem. J.264, 467.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - STOT RE 2 Oral
target_organs
Kidney
Classe de stockage
10 - Combustible liquids
wgk
WGK 1
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Cathepsin S: therapeutic, diagnostic, and prognostic potential
Wilkinson RDA, et al.
Biological Chemistry, 396(8), 867-882 (2015)
Cathepsin S activity regulates antigen presentation and immunity
Riese R J, et al.
The Journal of Clinical Investigation, 2351-2363 (1998)
Cathepsin S: therapeutic, diagnostic, and prognostic potential
Wilkinson RD, et al.
Applied Biological Chemistry, 396(8), 867-882 (2015)
Xiao-Yu Yuan et al.
Bioorganic & medicinal chemistry, 27(6), 1034-1042 (2019-02-19)
Selective proteinase inhibitors have demonstrated utility in the investigation of cartilage degeneration mechanisms and may have clinical use in the management of osteoarthritis. The cysteine protease cathepsin K (CatK) is an attractive target for arthritis therapy. Here we report the
Cathepsin S activity regulates antigen presentation and immunity
Riese RJ, et al.
The Journal of Clinical Investigation, 101(11), 2351-2363 (1998)
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