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A propos de cet article
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, mouse, rat
Application:
—
Citations:
23
Service technique
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Laissez-nous vous aiderbiological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Frozen Sections
Immunoblotting (1:100-1:300)
Immunoprecipitation (1:20-1:40)
Paraffin Sections (pretreatment required))
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
Gene Information
human ... COL10A1(1300)
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes type X collagen.
Recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen.
This Anti-Collagen Type X Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Collagen Type X.
Immunogen
Rat
purified, rat chondrosarcoma cell collagen type X
Application
Frozen Sections (see comments)
Immunoblotting (1:100-1:300)
Immunoprecipitation (1:20-1:40)
Paraffin Sections (see application reference; pretreatment required)
Immunoblotting (1:100-1:300)
Immunoprecipitation (1:20-1:40)
Paraffin Sections (see application reference; pretreatment required)
Physical form
Undiluted serum.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Chung, K.S., et al. 1995. Dev. Biol. 170, 387.
Schmid. T.M., and Linsenmayer, T.M. 1985. Dev. Biol. 107, 373.
Schmid. T.M., and Linsenmayer, T.M. 1985. Dev. Biol. 107, 373.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificats d'analyse (COA)
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Hypertrophic cartilage formation is a major setback in mesenchymal stem cells (MSCs)-mediated cartilage repair, and overcoming it requires optimization of differentiation. Here, we tested the miR-140 activated collagen hydrogel for the chondrogenic differentiation of MSCs and to produce hyaline cartilage.
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Nature communications, 8, 15019-15019 (2017-05-12)
Osteoarthritis (OA) is characterized by cartilage destruction and chondrocytes have a central role in this process. With age and inflammation chondrocytes have reduced capacity to synthesize and maintain ATP, a molecule important for cartilage homeostasis. Here we show that concentrations