Phosphatase Inhibitor Cocktail V

Phosphatase Inhibitor Cocktail Set V, 50X has been used:

• as a supplement in the lysis buffer to dissociate metabolically activated one (MMe) bone marrow macrophages (BMDMs) for Western blot
• in radio-immunoprecipitation assay (RIPA) modified buffer to incubate cultured human umbilical vein endothelial cells (HUVECs) for western blot
• in lysis buffer to lyse cultured HUVECs for immunoprecipitation
• in wash buffer to wash the protein complexes
• as a component in 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer to homogenize J774 cells to assess the effect of lipopolysaccharides (LPS) incubation on cytosolic phospholipase A2 (cPLA2) activity

Protein phosphorylation and dephosphorylation play a crucial role in signal transduction, controlling various cellular processes such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific serine, threonine, or tyrosine residues, which alters the protein′s conformation and its ability to interact with ligands. Protein phosphatases then remove the phosphate group, restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is of utmost importance to preserve their phosphorylation state.

Toxicity: Toxic (F)

A cocktail of four phosphatase inhibitors for the inhibition of both serine/threonine and protein tyrosine phosphatases. Available as 1 ml or as a set of five 1 ml vials.

Each vial contains 1 ml of aqueous solution with the following components:

• 250 mM Sodium Fluoride
• 50 mM ß-Glycerophosphate (Cat. No. 35675)
• 50 mM Sodium Pyrophosphate Decahydrate
• 50 mM Sodium Orthovanadate      

In H₂O.

Following initial thaw, aliquot and freeze (-20°C) for long-term storage. Dilute 1:50 just prior to use.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany