537088
Pronase® Protease, Streptomyces griseus, Nuclease-Free
Widely used broad-spectrum protease mixture
Synonyme(s) :
Nuclease-Free Protease, Streptomyces griseus, Pronase® Protease, Pronase® Protease, Streptomyces griseus
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A propos de cet article
Specific activity:
≥70,000 proteolytic units/g dry wt
Service technique
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RTECS - UK9595000
form
lyophilized
specific activity
≥70,000 proteolytic units/g dry wt
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: 10 mg/mL
foreign activity
Nuclease, none detected
shipped in
ambient
storage temp.
2-8°C
General description
PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.
Application
Pronase® Protease, Streptomyces griseus, nuclease-free has been used:
- in single-cell preparations for cardiac side population (CSP) analysis
- in aflatoxin plasma biomarker assay
- to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion
Biochem/physiol Actions
Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.
Preparation Note
Unstable in solution; reconstitute just prior to use.
Other Notes
Note: 1 KU = 1000 units.
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Classe de stockage
11 - Combustible Solids
wgk
WGK 2
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Ehsan Pourkarimi et al.
eLife, 5 (2016-12-24)
The primary task of developing embryos is genome replication, yet how DNA replication is integrated with the profound cellular changes that occur through development is largely unknown. Using an approach to map DNA replication at high resolution in C. elegans
Industrial Biotechnology and Commodity Products
Comprehensive Biotechnology, 3, 587-603 null
Annette Meeson et al.
Stem cell reviews and reports, 9(5), 731-739 (2013-04-27)
Cardiac side population (CSP) cells, defined by their ability to efflux the vital dye Hoechst 33342, have been identified as putative cardiac stem cells based on their potential to give rise to both cardiomyocytes and endothelial cells. The CSP phenotype