71341
pRSFDuet-1 DNA - Novagen
Synonyme(s) :
pRSFDuet-1 Vector
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A propos de cet article
NACRES:
NA.51
UNSPSC Code:
41106602
Promoter:
Promoter name: T7
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liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
promoter
Promoter name: T7
selection
kanamycin
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
pRSFDuet-1 is designed for the co-expression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (RBS). The vector also carries the pRSF1030 replicon (also known as NTP1), LacI gene, and kanamycin resistance marker.
Application
pRSFDuet-1 DNA - Novagen has been used as a vector:
- to clone the DNA fragments of defensin α 5 (DEFA5) for recombinant expression
- to clone the coding region of Mad1597–719
- to clone the globular domain of Bombyx mori Spc24 (73-162aa) with an N-terminal 6X His-tag to generate the B. mori 6xHis-Spc24(73-162)-Spc25(70-211) pRSF-DUET1 bacterial expression constructs
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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