AB5302
Anti-Amyloid Precursor Protein (APP) Antibody
CHEMICON®, rabbit polyclonal
Synonyme(s) :
APP
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A propos de cet article
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
monkey, mouse, human, bovine
Application:
ICC, IHC, IP, WB
Citations:
7
Service technique
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Anti-Amyloid Precursor Protein Antibody, KPI Domain, serum, Chemicon®
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
monkey, mouse, human, bovine
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... APP(351)
Immunogen
APP.
Application
Anti-Amyloid Precursor Protein Antibody, KPI Domain is an antibody against Amyloid Precursor Protein for use in IP, WB, IC, IH.
Western blot: 1:250 - 1:1,000
Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.
Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.
Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.
Optimal working dilutions must be determined by end user.
Reduction /Alkylation protocol:
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.
Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.
Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.
Optimal working dilutions must be determined by end user.
Reduction /Alkylation protocol:
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Biochem/physiol Actions
Amyloid Precursor Protein (APP), KPI Domain.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Classe de stockage
12 - Non Combustible Liquids
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In vivo regulation of amyloid precursor protein neuronal splicing by microRNAs.
Smith P, Al Hashimi A, Girard J, Delay C, Hebert SS.
Journal of Neurochemistry null
Jingdong Zhang et al.
Journal of neuroinflammation, 18(1), 100-100 (2021-04-28)
Microglia are resident innate immune cells in the brain, and activation of these myeloid cells results in secretion of a variety of pro-inflammatory molecules, leading to the development of neurodegenerative disorders. Lipopolysaccharide (LPS) is a widely used experimental stimulant in
Xinglong Yang et al.
Journal of biomedical research, 32(2), 136-144 (2018-01-24)
Microglia activation and white matter injury coexist after repeated episodes of mild brain trauma and ischemic stroke. Axon degeneration and demyelination can activate microglia; however, it is unclear whether early microglia activation can impair the function of white matter tracts
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| AB5302 | 04053252335037 |