Accéder au contenu
Merck

ABC80

Anti-phospho AMBRA1 Antibody (Ser52)

from rabbit, purified by affinity chromatography

Synonyme(s) :

Activating molecule in BECN1-regulated autophagy protein 1, Ambra, Ambra-1

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
WB, inhibition assay
Citations:
3

Principaux documents

Voir toute la documentation
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

inhibition assay: suitable (peptide), western blot: suitable

NCBI accession no.

NP_060219

UniProt accession no.

Q9C0C7

shipped in

wet ice

target post-translational modification

phosphorylation (pSer52)

Quality Level

100

Gene Information

human ... AMBRA1(55626)

General description

Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) interacts with BECN1, likely forming a complex with BECN1 and PIK3C3. AMBRA1 is involved in autophagy by controlling protein turnover during neuronal development, and in regulating differentiation, survival and proliferation of normal cells.
~150 kDa observed. Uniprot describes 6 isoforms from 83 kDa to 143 kDa This protein may be observed at higher molecular weights possibly due to hyperphosphorylation (Nazio, F., et al. (2013). Nat Cell Biol. [Epub ahead of print].).

Immunogen

Epitope: Phosphorylated Ser52
KLH-conjugated linear peptide corresponding to human AMBRA1 phosphorylated at Ser52.

Application

Detect Activating molecule in BECN1-regulated autophagy protein 1 using this rabbit polyclonal antibody, Anti-phospho AMBRA1 Antibody (Ser52) validated for use in western blotting & Peptide Inhibition Assay.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting/Peptide Inhibition Analysis: 2 µg/mL from a representative lot detected phospho AMBRA1 (Ser52) in WB/PIA.

Western Blotting Analysis: A representative lot from an independent laboratory detected phospho AMBRA1 (Ser52) in HEK293 cell lysates trated without Torin1 or EBS and demonstrated a loss of signal in HEK293 cell lysates treated with Torin1 or EBS (Nazio, F., et al. (2013). Nat Cell Biol.15(4):406-416.).

Biochem/physiol Actions

This antibody recognizes AMBRA1 phosphorylated at Ser52.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate
Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho AMBRA1 (Ser52) in 10 µg of HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Hui Liu et al.
Cellular and molecular biology (Noisy-le-Grand, France), 69(11), 139-245 (2023-11-28)
The research aims to explore the protective effects of ghrelin and its underlying molecular mechanisms in an H9C2 hypoxia/reoxygenation model. H9C2 cells were transfected with ghrelin overexpression lentiviral vector. The hypoxia/reoxygenation H9C2 model was constructed. The expression of ghrelin was
Zhengwei Ma et al.
The Journal of clinical investigation, 130(9), 5011-5026 (2020-08-18)
Dysregulation of autophagy in diabetic kidney disease (DKD) has been reported, but the underlying mechanism and its pathogenic role remain elusive. We show that autophagy was inhibited in DKD models and in human diabetic kidneys. Ablation of autophagy-related gene 7
Saverio Marchi et al.
EMBO molecular medicine, 7(11), 1403-1417 (2015-09-30)
Cerebral cavernous malformation (CCM) is a major cerebrovascular disease affecting approximately 0.3-0.5% of the population and is characterized by enlarged and leaky capillaries that predispose to seizures, focal neurological deficits, and fatal intracerebral hemorrhages. Cerebral cavernous malformation is a genetic

Numéro d'article de commerce international

RéférenceGTIN
ABC8004053252915987

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique