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Merck

ABT28

Anti-Autotaxin Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, WB
Citations:
2

Principaux documents

Voir toute la documentation
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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

canine (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

NCBI accession no.

NP_001035181

UniProt accession no.

Q13822

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ENPP2(5168)

General description

Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness.
~100/125 kDa observed.
3 isoforms are produced by alternative splicing with molecular weights of 99, 105, and 101 kDa The calculated molecular weight of this protein is 99 kDa, but can be observed at ~125 kDa due to high glycosylation. An uncharacterized band may appear at ~35 kDa in some lysates.

Immunogen

Epitope: Substrate binding region
KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region.

Application

Detect Autotaxin using this Anti-Autotaxin Antibody validated for use in WB, IH(P).
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed with Tween.

Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues.

Biochem/physiol Actions

This antibody recognizes Autotaxin at the substrate binding region.

Analysis Note

Control
Oligodendrocyte precursor cell lysate
Evaluated by Western Blot in oligodendrocyte precursor cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Paul A Volden et al.
Cancer prevention research (Philadelphia, Pa.), 9(5), 367-378 (2016-02-11)
Lysophosphatidic acid (LPA), acting in an autocrine or paracrine fashion through G protein-coupled receptors, has been implicated in many physiologic and pathologic processes, including cancer. LPA is converted from lysophosphatidylcholine (LPC) by the secreted phospholipase autotaxin (ATX). Although various cell
Filipa L Cardoso et al.
Journal of neuroinflammation, 12, 82-82 (2015-05-01)
The inflammatory mediator lipopolysaccharide (LPS) has been shown to induce acute gliosis in neonatal mice. However, the progressive effects on the murine neurodevelopmental program over the week that follows systemic inflammation are not known. Thus, we investigated the effects of

Numéro d'article de commerce international

RéférenceGTIN
ABT2804053252755934

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