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Merck

CBL434

Anti-c-Myc Tag Antibody

CHEMICON®, mouse monoclonal, 3C7

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A propos de cet article

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
3C7, monoclonal
Species reactivity:
mouse, human
Application:
ELISA, IP, WB
Citations:
6
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Nom du produit

Anti-c-myc Antibody, clone 3C7, clone 3C7, Chemicon®, from mouse

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

3C7, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYC(4609)
mouse ... Myc(17869)

Immunogen

Epitope: a.a. 171-188
Synthetic peptide: Cys-Ser-Thr-Ser-Ser-Leu-Tyr-Leu-Gln-Asp-Leu-Ser-Ala-Ala-Ala-Ser-Glu-Cys selected from the human c-myc sequence (amino acids (171-188). The peptide was conjugated to keyhole limpet haemocyanin.

Application

Detect c-myc also known as Proto-oncogene c-Myc with Anti-c-myc Antibody, clone 3C7 (Mouse Monoclonal Antibody), that has been demonstrated to work in ELISA, IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western blotting

Immunoprecipitation

Immunoassay detection of c-myc

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

In immunoblotting this antibody detects one major band at 62-64 kDa which can be blocked by pre-incubation with the immunizing peptide. In immunoprecipitation the antibody detects a 62 kDa band from cell lysates.

KNOWN SPECIES CROSS REACTIVITY: This antibody is known to cross react with mouse c-myc

Physical form

Format: Purified
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Preparation Note

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Chao Huang et al.
The Plant journal : for cell and molecular biology, 92(4), 546-556 (2017-08-30)
The pentatricopeptide repeat-DYW protein AtECB2 affects plastid RNA editing at seven sites, including accD-794, accD-1568, ndhF-290, ndhG-50, petL-5, rpoA-200 and rpoC1-488. To understand the mechanism of its involvement in RNA editing, a transgenic line was constructed with AtECB2 fused to
Lorena A Kallal et al.
SLAS discovery : advancing life sciences R & D, 26(2), 216-229 (2021-01-23)
While c-MYC is well established as a proto-oncogene, its structure and function as a transcription factor have made c-MYC a difficult therapeutic target. To identify small-molecule inhibitors targeting c-MYC for anticancer therapy, we designed a high-throughput screening (HTS) strategy utilizing
William B Tu et al.
Cancer cell, 34(4), 579-595 (2018-10-10)
MYC is an oncogenic driver that regulates transcriptional activation and repression. Surprisingly, mechanisms by which MYC promotes malignant transformation remain unclear. We demonstrate that MYC interacts with the G9a H3K9-methyltransferase complex to control transcriptional repression. Inhibiting G9a hinders MYC chromatin



Numéro d'article de commerce international

RéférenceGTIN
CBL43404053252477393