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Merck

CBL553

Anti-CD63 Antibody, clone RFAC4

clone RFAC4, Chemicon®, from mouse

Synonyme(s) :

Anti-LAMP-3, Anti-ME491, Anti-MLA1, Anti-OMA81H, Anti-TSPAN30

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
RFAC4, monoclonal
Application:
FACS, IHC (p), IP, WB
Citations:
35
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biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

RFAC4, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... CD63(967)

Catégories apparentées

Application

Anti-CD63 Antibody, clone RFAC4 is an antibody against CD63 for use in FC, IP, WB, IH, IH(P).
Flow cytometric studies of CD63 expression

Immunoprecipitation

Western blotting of CD63 antigen from solubilized platelet proteins

Suitable for formalin fixed paraffin wax embedded tissue sections. HIER and proteolytic pretreatment may not be necessary. Users should confirm specificity and pretreatment for their particular application.

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

This antibody reacts with a 53 kDa protein which form a part of the family of tetraspan moieties. The antigen was originally designated as a lysosomal membrane protein characterized as an activation dependent platelet surface antigen. In fact the CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma.

Physical form

Format: Purified
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Preparation Note

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Mariëtte E G Kranendonk et al.
Obesity (Silver Spring, Md.), 22(5), 1296-1308 (2013-12-18)
Extracellular vesicles (EVs) released by human adipocytes or adipose tissue (AT)-explants play a role in the paracrine interaction between adipocytes and macrophages, a key mechanism in AT inflammation, leading to metabolic complications like insulin resistance (IR) were determined. EVs released
Wiebke Petersen et al.
Molecular biology of the cell, 28(6), 726-735 (2017-01-27)
While lysosomes are degradative compartments and one of the defenses against invading pathogens, they are also hubs of metabolic activity. Late endocytic compartments accumulate around Plasmodium berghei liver-stage parasites during development, and whether this is a host defense strategy or
Haruka Shinohara et al.
Journal of immunology (Baltimore, Md. : 1950), 199(4), 1505-1515 (2017-07-12)
Macrophages are polarized into functional classically activated and alternatively activated (M2) phenotypes depending on their microenvironment, and these cells play an important role in the immune system. M2-like polarization of tumor-associated macrophages (TAMs) is activated by various secretions from cancer
Valentina Salvi et al.
JCI insight, 3(10) (2018-05-18)
The excessive production of type I IFNs is a hallmark and a main pathogenic mechanism of many autoimmune diseases, including systemic lupus erythematosus (SLE). In these pathologies, the sustained secretion of type I IFNs is dependent on the improper activation
Yonatan Perez et al.
Brain : a journal of neurology, 140(4), 928-939 (2017-03-24)
A novel autosomal recessive cerebro-renal syndrome was identified in consanguineous Bedouin kindred: neurological deterioration was evident as of early age, progressing into severe intellectual disability, profound ataxia, camptocormia and oculomotor apraxia. Brain MRI was normal. Four of the six affected

Numéro d'article de commerce international

RéférenceGTIN
CBL55304053252619212

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