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Merck

CP06

Anti-α-Tubulin Mouse mAb (DM1A)

liquid, clone DM1A, Calbiochem®

Synonyme(s) :

Mouse Anti-α-Tubulin, α-Tubulin Detection Antibody, Anti-Tubulin DM1A

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Nom du produit

Anti-α-Tubulin Mouse mAb (DM1A), liquid, clone DM1A, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

DM1A, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, gerbil, chicken, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Immunoblotting (20-100 ng/mL, colorimetric or chemiluminescence;
Immunofluorescence (5 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TUBA1A(7846)

Analysis Note

Negative Control
trp E (Ab-1)
Positive Control
Any cell line

Application

Immunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)

Immunofluorescence (5 µg/ml)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.
Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.
This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.

Immunogen

Chicken
native chick brain microtubules

Other Notes

Blose, S.H., et al. 1984. J. Cell. Biol.98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Tubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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