MAB1340
Anti-Collagen Type I (COL1A1) Antibody
CHEMICON®, mouse monoclonal, C11
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A propos de cet article
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
C11, monoclonal
Species reactivity:
human
Application:
ELISA, IHC
Citations:
11
Service technique
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Anti-Collagen Type I Antibody, clone C11, ascites fluid, clone C11, Chemicon®
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
C11, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... COL1A1(1277)
Application
Detect Collagen Type I using this Anti-Collagen Type I Antibody, clone C11 validated for use in ELISA, IH.
Immunohistochemical staining of 10 μm thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is not recommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.
ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.
Optimal working dilutions must be determined by the end user.
ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and II in ELISA, RIA or immunoblotting.
Physical form
Immunoglobulin Purified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose. . Lyophilized, reconstitute with 1 mL of distilled water.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificats d'analyse (COA)
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Zoumi, A; Yeh, A; Tromberg, BJ
Proceedings of the National Academy of Sciences of the USA null
Jose M Gonzalez et al.
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Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| MAB1340 | 04053252358852 |