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Merck

MAB19292

Anti-ADAM2 Antibody, clone 9D2.2

clone 9D2.2, Chemicon®, from mouse

Synonyme(s) :

Fertilin beta

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
9D2.2, monoclonal
Application:
ELISA, WB
Citations:
15
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9D2.2, monoclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, western blot: suitable

isotype

IgG2b

suitability

not suitable for immunohistochemistry

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ADAM2(2515)

Immunogen

Recombinant mouse fertilin produced by baculovirus expressin in SF9 cells.

Application

Research Category
Cell Structure
Research Sub Category
ECM Proteins

MMPs & TIMPs
This Anti-ADAM2 Antibody, clone 9D2.2 is validated for use in ELISA, WB for the detection of ADAM2.
Western blot: 1:500 - 1:1,000 dilution for detected against sperm lysates, using standard alkaline phosphatase with NBT/BCIP detection. Chemiluminescent detection may permit higher dilutions.

Not effective for immunohistochemistry.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes mouse fertilin beta by immunoblot, staining of bands in a 46-48 kDa range (multiple bands are due to variable protein glycosylation).

Physical form

Format: Purified
Liquid at 1 mg/mL in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Consulter la Bibliothèque de documents



Ryo Yamaguchi et al.
Biology of reproduction, 75(5), 760-766 (2006-07-28)
Male mice deficient for the calmegin (Clgn) or the angiotensin-converting enzyme (Ace) gene show impaired sperm migration into the oviduct and loss of sperm-zona pellucida binding ability in vitro. Since CLGN is a molecular chaperone for membrane transport of target
Daiji Kiyozumi et al.
Biology of reproduction, 109(4), 474-481 (2023-08-02)
The mammalian epididymis is the organ for functional sperm maturation. In rodents, the initial segment, the most proximal region of the epididymis, plays a critical role in sperm maturation. The luminal epithelial differentiation and the following gene expression of the
Ryo Yamaguchi et al.
Genes to cells : devoted to molecular & cellular mechanisms, 13(8), 851-861 (2008-09-11)
CD52 is a glycosylphosphatidylinositol (GPI)-anchored antigen expressed on lymphocytes and in epididymal epithelial cells. CD52 is also known as "maturation-associated sperm antigen" but its function is unknown. We therefore generated Cd52 disrupted mice. The resulting Cd52 null mice were healthy



Numéro d'article de commerce international

RéférenceGTIN
MAB1929204053252265778