MAB2019Z
Anti-Integrin αVβ5 Antibody
CHEMICON®, mouse monoclonal, 15F11
Synonyme(s) :
MAB2019
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A propos de cet article
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
15F11, monoclonal
Species reactivity:
human
Application:
ELISA, FACS, ICC, IP
Citations:
15
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Laissez-nous vous aiderNom du produit
Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free, clone 15F11, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
15F11, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ITGAV(3685)
Catégories apparentées
Immunogen
Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.
Application
Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free is an antibody against Integrin αVβ5 for use in ELISA, FC, IP & IC.
Immunoprecipitation
Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X10E5 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.
Immunocytochemistry: 15μg/mL dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation with MAB2019Z antibody for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.
ELISA
Optimal working dilutions must be determined by end user.
Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X10E5 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.
Immunocytochemistry: 15μg/mL dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation with MAB2019Z antibody for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Biochem/physiol Actions
Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. The antibody binds to the alphaVbeta5 complex. The monoclonal antibody MAB2019Z has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding of the antibody to alphaVbeta5. Monoclonal antibody MAB2019Z does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
Physical form
Format: Purified
Purified immunoglobulin. Liquid at 1 mg/mL in PBS, containing no preservatives.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificats d'analyse (COA)
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Stimulation of glioma cell motility by expression, proteolysis, and release of the L1 neural cell recognition molecule.
Yang, M; Adla, S; Temburni, MK; Patel, VP; Lagow, EL; Brady, OA; Tian, J; Boulos, MI; Galileo, DS
Cancer Cell International null
Khine Kyaw Oo et al.
BMC cancer, 21(1), 65-65 (2021-01-16)
Chemoresistance is one of the main problems in treatment of cancer. Periostin (PN) is a stromal protein which is mostly secreted from cancer associated fibroblasts in the tumor microenvironment and can promote cancer progression including cell survival, metastasis, and chemoresistance.
Daniel Vial et al.
Journal of cell science, 121(Pt 10), 1661-1670 (2008-05-01)
The plasminogen activation system regulates matrix remodeling through both proteolytic and non-proteolytic mechanisms. Studies were undertaken to determine the effects of the plasminogen activator inhibitor 1 (PAI1) on the assembly of the fibronectin matrix. The addition of PAI1 to MG-63
Hongquan Zhang et al.
The Journal of cell biology, 158(7), 1287-1297 (2002-10-03)
p21-activated kinase 1 (PAK1) can affect cell migration (Price et al., 1998; del Pozo et al., 2000) and modulate myosin light chain kinase and LIM kinase, which are components of the cellular motility machinery (Edwards, D.C., L.C. Sanders, G.M. Bokoch
Aliakbar Taherian et al.
BMC cancer, 11, 293-293 (2011-07-15)
Integrins are used as prognostic indicators in breast cancer. Following engagement with extracellular matrix proteins, their signaling influences numerous cellular processes including migration, proliferation, and death. Integrin signaling varies between cell types through differential expression of integrin subunits, and changes
Contenu apparenté
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| MAB2019Z | 04053252577789 |
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