MAB365
Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG
clone 192-IgG, Chemicon®, from mouse
Synonyme(s) :
CD271 antigen, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, low affinity nerve growth factor receptor, nerve growth factor receptor, nerve growth factor receptor (TNFR superfamily, member 16), p75 IC
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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
192-IgG, monoclonal
Application:
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
rat
Citations:
53
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
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Laissez-nous vous aiderNom du produit
Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG, clone 192-IgG, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
192-IgG, monoclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NGFR(4804)
Analysis Note
Control
Western Blot: NGF-differentiated PC12 cell lysate.
Immunohistochemistry: Rat brain tissue.
Western Blot: NGF-differentiated PC12 cell lysate.
Immunohistochemistry: Rat brain tissue.
Immunohistochemistry:
1-5 µg/mL on rat , of a previous lot, (spinal chord, see protocol below); other fixatives and methods can also be used.
1-5 µg/mL on rat , of a previous lot, (spinal chord, see protocol below); other fixatives and methods can also be used.
Application
Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG is an antibody against Nerve Growth Factor Receptor for use in IC, IH, IP, FUNC & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
Western Blot:
1-5 µg/mL (Non-reducing conditions only) was used on a previous lot.
Inhitibition Assay:
Inhibition on NGF binding {Chandler, 1984}.
Immunoprecipitation:
5 µg/mL, 0.5% triton X-100 buffer; PC12 positive control was used on a previous lot.
Immunohistochemistry:
1-5 µg/mL on rat spinal chord, of a previous lot was used. Other fixatives and methods can also be used.
1-5 µg/mL (Non-reducing conditions only) was used on a previous lot.
Inhitibition Assay:
Inhibition on NGF binding {Chandler, 1984}.
Immunoprecipitation:
5 µg/mL, 0.5% triton X-100 buffer; PC12 positive control was used on a previous lot.
Immunohistochemistry:
1-5 µg/mL on rat spinal chord, of a previous lot was used. Other fixatives and methods can also be used.
Biochem/physiol Actions
Reacts with rat p75 protein.
Specific for the rat low affinity Nerve Growth Factor-Receptor (p75), does not react with human or mouse p75.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
75 kDa
The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells.
Immunogen
Epitope: Extracellular
n-octoglucoside stabilized proteins containing p75 receptor, which were isolated from plasma membranes of PC-12 cells (Chandler et al., 1984).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1111
Physical form
Format: Purified
Mouse monoclonal IgG1 in 0.02M Phosphate buffer, pH7.6, 0.25M NaCl with 0.1% sodium azide
Protein A purified
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Classe de stockage
10 - Combustible liquids
wgk
WGK 2
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Marta Volosin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(39), 9870-9879 (2008-09-26)
Seizure-induced damage elicits a loss of hippocampal neurons mediated to a great extent by the p75 neurotrophin receptor (NTR). Proneurotrophins, which are potent apoptosis-inducing ligands for p75(NTR), were increased in the hippocampus, particularly in astrocytes, by pilocarpine-induced seizures; and infusion
Ying Li et al.
Cell transplantation, 25(2), 293-299 (2015-07-02)
Cell transplantation is one of the most promising strategies for repair of human spinal cord injuries. Animal studies from a number of laboratories have shown that transplantation of olfactory ensheathing cells cultured from biopsies of the olfactory bulb mediate axonal
Mary Ann Asson-Batres et al.
The Journal of comparative neurology, 496(2), 149-171 (2006-03-16)
Work from our laboratory suggests that retinoic acid (RA) influences neuron development in the postnatal olfactory epithelium (OE). The studies reported here were carried out to identify and localize retinaldehyde dehydrogenase (RALDH) expression in postnatal rat OE to gain a
A Petersen et al.
Nature communications, 9(1), 4430-4430 (2018-10-27)
Biomaterials developed to treat bone defects have classically focused on bone healing via direct, intramembranous ossification. In contrast, most bones in our body develop from a cartilage template via a second pathway called endochondral ossification. The unsolved clinical challenge to
Tara Lovekamp-Swan et al.
Brain research, 1159, 54-66 (2007-06-22)
Estrogen is a powerful neuroprotective agent with the ability to induce trophic and antiapoptotic genes. However, concerns about negative overall health consequences of estrogen replacement after menopause have led to the adoption of other strategies to obtain estrogen's benefits in
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| MAB365 | 04053252727252 |
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