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Merck

MAB3864

Anti-DNA/Histone H1 Antibody

Chemicon®, from mouse

Synonyme(s) :

Anti-H1.4, Anti-H1E, Anti-H1F4, Anti-H1s-4, Anti-HIST1H1E, Anti-RMNS, Anti-dJ221C16.5

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
monoclonal
Application:
ELISA, FACS, ICC, IHC
Citations:
39
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biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... H1-1(3024)

Catégories apparentées

Immunogen

Human Raji cell nuclei

Application

Anti-DNA/Histone H1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of DNA/Histone H1 & has been validated in ELISA, FC, ICC & IHC.
Immunocytochemistry: 5-10 μg/mL on 2% paraformaldehyde fixed cells (10 minutes at room temperature)

Immunohistochemistry: 5-10 μg/mL on paraformaldehyde or B5 fixed tissue sections.

Flow cytometry: 5-10 μg/mL using 2% paraformaldehyde fixed cells.

ELISA, direct and indirect

Optimal working dilutions must be determined by end user.

STAINING PATTERN:

Nuclear staining observed. Dense staining of nuclei. No staining of nucleoli.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Biochem/physiol Actions

Reacts with DNA/Histone 1. The antibody can be used to stain nuclei of all species and to identify necrosis. By immunohistochemistry, the antibody shows dense staining of nuclei with no staining of nucleoli observed.

Physical form

Format: Purified
Protein A purified culture supernatant. Liquid in PBS

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Neila Álvarez de Haro et al.
Fish & shellfish immunology, 119, 209-219 (2021-08-27)
Neutrophils release chromatin extracellular traps (ETs) as part of the fish innate immune response to counter the threats posed by microbial pathogens. However, relatively little attention has been paid to this phenomenon in many commercially farmed species, despite the importance
Nicole de Buhr et al.
Cellular microbiology, 19(2) (2016-07-28)
Streptococcus suis is an important meningitis-causing pathogen in pigs and humans. Neutrophil extracellular traps (NETs) have been identified as host defense mechanism against different pathogens. Here, NETs were detected in the cerebrospinal fluid (CSF) of S. suis-infected piglets despite the presence
Gang Liu et al.
Mucosal immunology, 12(4), 862-873 (2019-04-13)
Extra-intestinal manifestations (EIM) are common in inflammatory bowel disease (IBD). One such EIM is sub-clinical pulmonary inflammation, which occurs in up to 50% of IBD patients. In animal models of colitis, pulmonary inflammation is driven by neutrophilic infiltrations, primarily in
Nicole de Buhr et al.
Frontiers in microbiology, 9, 966-966 (2018-06-06)
Granulocytes mediate the first line of defense against infectious diseases in humans as well as animals and they are well known as multitasking cells. They can mediate antimicrobial activity by different strategies depending on the pathogen they encounter. Besides phagocytosis
Neutrophil Responses to Sterile Implant Materials.
Jhunjhunwala, S; Aresta-DaSilva, S; Tang, K; Alvarez, D; Webber, MJ; Tang, BC; Lavin et al.
Testing null

Numéro d'article de commerce international

RéférenceGTIN
MAB386404053252612503

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