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UNSPSC Code:
12352207
NACRES:
NA.54
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Laissez-nous vous aiderbiological source
human serum (transferrin), synthetic (sodium selenite)
recombinant
expressed in human cells
sterility
non-sterile; 0.2 μm filtered
assay
>98% (Transferrin, SDS-PAGE), >99% (Sodium selenite)
Gene Information
bovine ... INS(280829)
human ... TFRC(7037)
form
lyophilized
packaging
pkg of 50 mg (for 5 l medium)
manufacturer/tradename
Roche
technique(s)
cell culture | mammalian: suitable
solubility
water: miscible
storage temp.
2-8°C
General description
The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.
Application
The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.
Preparation Note
Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)
Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.
Further dilution with culture medium.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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